Survival of Staphylococcus epidermidis in Fibroblasts and Osteoblasts.
Infect Immun
; 86(10)2018 10.
Article
en En
| MEDLINE
| ID: mdl-30061380
ABSTRACT
Staphylococcus epidermidis is a leading cause of infections associated with indwelling medical devices, including prosthetic joint infection. While biofilm formation is assumed to be the main mechanism underlying the chronic infections S. epidermidis causes, we hypothesized that S. epidermidis also evades immune killing, contributing to its pathogenesis. Here, we show that prosthetic joint-associated S. epidermidis isolates can persist intracellularly within human fibroblasts and inside human and mouse osteoblasts. We also show that the intracellularly persisting bacteria reside primarily within acidic phagolysosomes and that over the course of infection, small-colony variants are selected for. Moreover, upon eukaryotic cell death, these bacteria, which can outlive their host, can escape into the extracellular environment, providing them an opportunity to form biofilms on implant surfaces at delayed time points in implant-associated infection. In summary, the acidic phagolysosomes of fibroblasts and osteoblasts serve as reservoirs for chronic or delayed S. epidermidis infection.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Osteoblastos
/
Infecciones Estafilocócicas
/
Staphylococcus epidermidis
/
Infecciones Relacionadas con Prótesis
/
Biopelículas
/
Fibroblastos
/
Membranas Intracelulares
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Infect Immun
Año:
2018
Tipo del documento:
Article
País de afiliación:
Estados Unidos