Deletion of Stearoyl-CoA Desaturase-1 From the Intestinal Epithelium Promotes Inflammation and Tumorigenesis, Reversed by Dietary Oleate.
Gastroenterology
; 155(5): 1524-1538.e9, 2018 11.
Article
en En
| MEDLINE
| ID: mdl-30063922
ABSTRACT
BACKGROUND & AIMS:
The enzyme stearoyl-coenzyme A desaturase 1 (SCD or SCD1) produces monounsaturated fatty acids by introducing double bonds into saturated bonds between carbons 9 and 10, with oleic acid as the main product. SCD1 is present in the intestinal epithelium, and fatty acids regulate cell proliferation, so we investigated the effects of SCD1-induced production of oleic acid in enterocytes in mice.METHODS:
We generated mice with disruption of Scd1 selectively in the intestinal epithelium (iScd1-/- mice) on a C57BL/6 background; iScd1+/+ mice were used as controls. We also generated iScd1-/-ApcMin/+ mice and studied cancer susceptibility. Mice were fed a chow, oleic acid-deficient, or oleic acid-rich diet. Intestinal tissues were collected and analyzed by histology, reverse transcription quantitative polymerase chain reaction, immunohistochemistry, and mass spectrometry, and tumors were quantified and measured.RESULTS:
Compared with control mice, the ileal mucosa of iScd1-/- mice had a lower proportion of palmitoleic (C161 n-7) and oleic acids (C181 n-9), with accumulation of stearic acid (C180); this resulted a reduction of the Δ9 desaturation ratio between monounsaturated (C161 n-7 and C181 n-9) and saturated (C160 and C180) fatty acids. Ileal tissues from iScd1-/- mice had increased expression of markers of inflammation activation and crypt proliferative genes compared with control mice. The iScd1-/-ApcMin/+ mice developed more and larger tumors than iScd1+/+ApcMin/+ mice. iScd1-/-ApcMin/+ mice fed the oleic acid-rich diet had reduced intestinal inflammation and significantly lower tumor burden compared with mice fed a chow diet.CONCLUSIONS:
In studies of mice, we found intestinal SCD1 to be required for synthesis of oleate in the enterocytes and maintenance of fatty acid homeostasis. Dietary supplementation with oleic acid reduces intestinal inflammation and tumor development in mice.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Estearoil-CoA Desaturasa
/
Grasas Insaturadas en la Dieta
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Ácido Oléico
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Enteritis
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Mucosa Intestinal
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Neoplasias Intestinales
Límite:
Animals
Idioma:
En
Revista:
Gastroenterology
Año:
2018
Tipo del documento:
Article
País de afiliación:
Italia