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Neuronal-Specific TUBB3 Is Not Required for Normal Neuronal Function but Is Essential for Timely Axon Regeneration.
Latremoliere, Alban; Cheng, Long; DeLisle, Michelle; Wu, Chen; Chew, Sheena; Hutchinson, Elizabeth B; Sheridan, Andrew; Alexandre, Chloe; Latremoliere, Frederic; Sheu, Shu-Hsien; Golidy, Sara; Omura, Takao; Huebner, Eric A; Fan, Yanjie; Whitman, Mary C; Nguyen, Elaine; Hermawan, Crystal; Pierpaoli, Carlo; Tischfield, Max A; Woolf, Clifford J; Engle, Elizabeth C.
Afiliación
  • Latremoliere A; Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA, USA; Department of Neurobiology, Harvard Medical School, Boston, MA, USA.
  • Cheng L; Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA, USA; Department of Neurology, Boston Children's Hospital, Boston, MA, USA; Department of Neurology, Harvard Medical School, Boston, MA, USA.
  • DeLisle M; Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA, USA; Department of Neurology, Boston Children's Hospital, Boston, MA, USA; Howard Hughes Medical Institute, Chevy Chase, MD, USA.
  • Wu C; Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA, USA; Department of Neurology, Boston Children's Hospital, Boston, MA, USA; Department of Neurology, Harvard Medical School, Boston, MA, USA.
  • Chew S; Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA, USA; Department of Neurology, Boston Children's Hospital, Boston, MA, USA; Howard Hughes Medical Institute, Chevy Chase, MD, USA.
  • Hutchinson EB; Quantitative Medical Imaging Section, National Institute of Biomedical Imaging and Bioengineering, NIH, Bethesda, MD, USA; The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD, USA.
  • Sheridan A; Department of Neurology, Boston Children's Hospital, Boston, MA, USA.
  • Alexandre C; Department of Neurology, Beth Israel Deaconess Medical Center, Harvard Medical School, Boston, MA, USA.
  • Latremoliere F; Department of Mathematics, University of Denver, Denver, CO, USA.
  • Sheu SH; Department of Pathology and Department of Cardiology, Boston Children's Hospital, Boston, MA, USA.
  • Golidy S; Department of Neurology, Boston Children's Hospital, Boston, MA, USA; Howard Hughes Medical Institute, Chevy Chase, MD, USA.
  • Omura T; Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA, USA; Department of Neurobiology, Harvard Medical School, Boston, MA, USA; Department of Orthopedic Surgery, Hamamatsu University School of Medicine, Hamamatsu, Japan.
  • Huebner EA; Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA, USA; Department of Neurobiology, Harvard Medical School, Boston, MA, USA.
  • Fan Y; Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA, USA; Department of Neurology, Boston Children's Hospital, Boston, MA, USA; Department of Neurology, Harvard Medical School, Boston, MA, USA.
  • Whitman MC; Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA, USA; Department of Ophthalmology, Boston Children's Hospital, Boston, MA, USA; Department of Ophthalmology, Harvard Medical School, Boston, MA, USA.
  • Nguyen E; Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA, USA; Department of Ophthalmology, Boston Children's Hospital, Boston, MA, USA.
  • Hermawan C; Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA, USA; Department of Neurology, Boston Children's Hospital, Boston, MA, USA.
  • Pierpaoli C; Quantitative Medical Imaging Section, National Institute of Biomedical Imaging and Bioengineering, NIH, Bethesda, MD, USA; The Henry M. Jackson Foundation for the Advancement of Military Medicine, Inc., Bethesda, MD, USA.
  • Tischfield MA; Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA, USA; Department of Neurology, Boston Children's Hospital, Boston, MA, USA; Department of Neurology, Harvard Medical School, Boston, MA, USA.
  • Woolf CJ; Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA, USA; Department of Neurobiology, Harvard Medical School, Boston, MA, USA.
  • Engle EC; Kirby Neurobiology Center, Boston Children's Hospital, Boston, MA, USA; Department of Neurology, Boston Children's Hospital, Boston, MA, USA; Department of Ophthalmology, Boston Children's Hospital, Boston, MA, USA; Department of Neurology, Harvard Medical School, Boston, MA, USA; Department of Opht
Cell Rep ; 24(7): 1865-1879.e9, 2018 08 14.
Article en En | MEDLINE | ID: mdl-30110642
ABSTRACT
We generated a knockout mouse for the neuronal-specific ß-tubulin isoform Tubb3 to investigate its role in nervous system formation and maintenance. Tubb3-/- mice have no detectable neurobehavioral or neuropathological deficits, and upregulation of mRNA and protein of the remaining ß-tubulin isotypes results in equivalent total ß-tubulin levels in Tubb3-/- and wild-type mice. Despite similar levels of total ß-tubulin, adult dorsal root ganglia lacking TUBB3 have decreased growth cone microtubule dynamics and a decreased neurite outgrowth rate of 22% in vitro and in vivo. The effect of the 22% slower growth rate is exacerbated for sensory recovery, where fibers must reinnervate the full volume of the skin to recover touch function. Overall, these data reveal that, while TUBB3 is not required for formation of the nervous system, it has a specific role in the rate of peripheral axon regeneration that cannot be replaced by other ß-tubulins.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Tubulina (Proteína) / Isoformas de Proteínas / Proyección Neuronal / Regeneración Nerviosa Límite: Animals Idioma: En Revista: Cell Rep Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Tubulina (Proteína) / Isoformas de Proteínas / Proyección Neuronal / Regeneración Nerviosa Límite: Animals Idioma: En Revista: Cell Rep Año: 2018 Tipo del documento: Article País de afiliación: Estados Unidos