Studies on inorganic pyrophosphatase using imidodiphosphate as a substrate.
FEBS Lett
; 206(1): 121-4, 1986 Sep 29.
Article
en En
| MEDLINE
| ID: mdl-3019764
ABSTRACT
Baker's yeast inorganic pyrophosphatase has been found to catalyze Mg2+-dependent hydrolysis of imidodiphosphate yielding phosphate and amidophosphate. The reaction proceeds linearly in the presteady state. The catalytic constant is maximal at pH 9.0 and equals 0.5 min-1. Kinetic titrations of the enzyme with imidodiphosphate and Mg2+ have provided direct evidence for the involvement of three Mg2+ per active site in the transition state of the pyrophosphatase reaction.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Pirofosfatasas
/
Saccharomyces cerevisiae
/
Difosfonatos
Idioma:
En
Revista:
FEBS Lett
Año:
1986
Tipo del documento:
Article