Development of 12 microsatellite markers for Bombina orientails based on RNA-Seq and their usefulness in population genetic diversity.
Mol Biol Rep
; 45(6): 2811-2814, 2018 Dec.
Article
en En
| MEDLINE
| ID: mdl-30203239
ABSTRACT
Bombina orientalis is widely used due to bombesin which isolated from its skin. But in recent years, the population of B. orientalis has become declining distinctly because of human activities, environmental pollution, drought climatic conditions and other factors. In order to provide the molecular basis for the proposal of biodiversity conservation, we report the development of 12 microsatellite markers for B. orientalis based on RNA-Seq. We test polymorphism against in 48 B. orientalis individuals which randomly selected from 182 individuals take advantage of polyacrylamide gel electrophoresis (PAGE). These markers will be useful in the research on the genetic diversity, population genetic structure and other studies. For B. orientalis, all of these loci showed polymorphism, and in line with the H-W equilibrium law. The number of alleles per locus ranged from 3 to 21. The observed and expected heterozygosities ranged from 0.0118 to 0.7795 and from 0.1612 to 0.8703, respectively. The polymorphism information content ranged from 0.153 to 0.857. And the genetic diversity of B. orientalis in Lushui Rivers is significantly higher than that in the Maoer Mountains.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Anuros
/
Repeticiones de Microsatélite
Límite:
Animals
País/Región como asunto:
Asia
Idioma:
En
Revista:
Mol Biol Rep
Año:
2018
Tipo del documento:
Article
País de afiliación:
China