Phenotypic and genotypic analyses of antimicrobial resistant bacteria in livestock in Uganda.
Transbound Emerg Dis
; 66(1): 317-326, 2019 Jan.
Article
en En
| MEDLINE
| ID: mdl-30260584
ABSTRACT
Antimicrobial resistant bacteria (ARB) in livestock are a global public health concern, not only because they prolong infectious diseases but also they can be transferred from animals to humans via the food chain. Here, we studied ARB in livestock at commercial and subsistence farms (n = 13) in Wakiso and Mpigi districts, Uganda. We enquired from the farmers about the type and the purpose of antimicrobial agents they have used to treat their livestock. After collecting faeces, we isolated antimicrobial resistant Escherichia coli from livestock faeces (n = 134) as an indicator bacterium. These strains showed resistance to ampicillin (44.8%), tetracycline (97.0%), and sulfamethoxazole-trimethoprim (56.7%). The frequency of ampicillin-resistance was significantly correlated with the usage of penicillins to livestock in the farms (p = 0.04). The metagenomics data detected 911 antimicrobial resistant genes that were classified into 16 categories. Genes for multidrug efflux pumps were the most prevalent category in all except in one sample. Interestingly, the genes encoding third-generation cephalosporins (blaCTX-M ), carbapenems (blaACT ), and colistin (arnA) were detected by metagenomics analysis although these phenotypes were not detected in our E. coli strains. Our results suggest that the emergence and transmission of cephalosporin, carbapenem, and/or colistin-resistant bacteria among livestock can occur in future if these antimicrobial agents are used.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Enfermedades de las Aves de Corral
/
Pollos
/
Farmacorresistencia Bacteriana
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Escherichia coli
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Infecciones por Escherichia coli
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Ganado
Tipo de estudio:
Prevalence_studies
/
Risk_factors_studies
Límite:
Animals
País/Región como asunto:
Africa
Idioma:
En
Revista:
Transbound Emerg Dis
Asunto de la revista:
MEDICINA VETERINARIA
Año:
2019
Tipo del documento:
Article
País de afiliación:
Japón