Substrate and inhibitor selectivity, and biological activity of an epoxide hydrolase from Trichoderma reesei.
Mol Biol Rep
; 46(1): 371-379, 2019 Feb.
Article
en En
| MEDLINE
| ID: mdl-30426381
ABSTRACT
Epoxide hydrolases (EHs) are present in all living organisms and catalyze the hydrolysis of epoxides to the corresponding vicinal diols. EH are involved in the metabolism of endogenous and exogenous epoxides, and thus have application in pharmacology and biotechnology. In this work, we describe the substrates and inhibitors selectivity of an epoxide hydrolase recently cloned from the filamentous fungus Trichoderma reesei QM9414 (TrEH). We also studied the TrEH urea-based inhibitors effects in the fungal growth. TrEH showed high activity on radioative and fluorescent surrogate and natural substrates, especially epoxides from docosahexaenoic acid. Using a fluorescent surrogate substrate, potent inhibitors of TrEH were identified. Interestingly, one of the best compounds inhibit up to 60% of T. reesei growth, indicating an endogenous role for TrEH. These data make TrEH very attractive for future studies about fungal metabolism of fatty acids and possible development of novel drugs for human diseases.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Trichoderma
/
Epóxido Hidrolasas
Idioma:
En
Revista:
Mol Biol Rep
Año:
2019
Tipo del documento:
Article
País de afiliación:
Brasil