CD133 promotes the self-renewal capacity of thyroid cancer stem cells through activation of glutamate aspartate transporter SLC1A3 expression.
Biochem Biophys Res Commun
; 511(1): 87-91, 2019 03 26.
Article
en En
| MEDLINE
| ID: mdl-30771902
ABSTRACT
CD133+ cancer stem cells are responsible for thyroid cancer initiation. The regulatory pathways essential for sustaining the self-renewal of thyroid cancer stem cells remain largely unknown. Glutamate signaling regulates the self-renewal ability of stem cells. In the present study, we found that the level of glutamate was higher in CD133+ thyroid cancer cells than in CD133- thyroid cancer cells. The transcriptional level of glutamate aspartate transporter SLC1A3 was high in CD133+ thyroid cancer cells. Activation of NF-κB signaling by CD133 was responsible for SLC1A3 high transcription level in CD133+ thyroid cancer cells. Knock down of SLC1A3 significantly reduced the level of glutamate and inhibited the self-renewal activity and tumorigenicity of CD133+ thyroid cancer cells. Overexpression of SLC1A3 rescued the negative effect of CD133 knockdown on the self-renewal capability of CD133+ thyroid cancer cells. Taken together, CD133 promotes the self-renewal capacity of CD133+ thyroid cancer cells at least partly through activation of SLC1A3 expression.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Células Madre Neoplásicas
/
Neoplasias de la Tiroides
/
Transportador 1 de Aminoácidos Excitadores
/
Autorrenovación de las Células
/
Antígeno AC133
Límite:
Humans
Idioma:
En
Revista:
Biochem Biophys Res Commun
Año:
2019
Tipo del documento:
Article
País de afiliación:
China