Macropinocytosis contributes to hantavirus entry into human airway epithelial cells.
Virology
; 531: 57-68, 2019 05.
Article
en En
| MEDLINE
| ID: mdl-30852272
ABSTRACT
Hantaviruses are emerging rodent-borne negative-strand RNA viruses associated with severe human diseases. Zoonotic transmission occurs via aerosols of contaminated rodent excreta and cells of the human respiratory epithelium represent likely early targets. Here we investigated cellular factors involved in entry of the pathogenic Old and New World hantaviruses Hantaan virus (HTNV) and Andes virus (ANDV) into human respiratory epithelial cells. Screening of a kinase inhibitor library using a biocontained recombinant vesicular stomatitis virus pseudotype platform revealed differential requirement for host kinases for HTNV and ANDV entry and provided first hints for an involvement of macropinocytosis. Examination of a selected panel of well-defined inhibitors of endocytosis confirmed that both HTNV and ANDV enter human respiratory epithelial cells via a pathway that critically depends on sodium proton exchangers and actin, hallmarks of macropinocytosis. However, HTNV and ANDV differed in their individual requirements for regulatory factors of macropinocytosis, indicating virus-specific differences.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Orthohantavirus
/
Infecciones por Hantavirus
/
Mucosa Respiratoria
/
Endocitosis
/
Células Epiteliales
/
Internalización del Virus
Límite:
Humans
Idioma:
En
Revista:
Virology
Año:
2019
Tipo del documento:
Article
País de afiliación:
Suiza