Bioluminescent aptamer-based solid-phase microassay to detect lung tumor cells in plasma.
Talanta
; 199: 674-678, 2019 Jul 01.
Article
en En
| MEDLINE
| ID: mdl-30952314
ABSTRACT
Two high-affinity DNA aptamers for lung tumor cells were applied as biospecific elements in bioluminescent assay of patient blood. The oligonucleotide complementary to the 5' end of both aptamers carrying either biotin or Ca2+-regulated photoprotein obelin was used to form a sandwich-type analytical complex on the surfaces of magnetic streptavidin-activated microspherical particles. Clinical blood samples from cases of morphologically confirmed lung cancer and control samples were analyzed applying the developed assay. From the receiver operator curve (ROC) analysis, the chosen threshold value as clinical decision limit offers the sensitivity of 91.5% and the specificity of 75% (pâ¯<â¯0.001). The area under ROC curve with the value of 0.901 distinguishes well between the two groups under investigation.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Aptámeros de Nucleótidos
/
Mediciones Luminiscentes
/
Neoplasias Pulmonares
Tipo de estudio:
Prognostic_studies
Límite:
Humans
Idioma:
En
Revista:
Talanta
Año:
2019
Tipo del documento:
Article
País de afiliación:
Rusia