An organic polymer monolith modified with an amino acid ionic liquid and graphene oxide for use in capillary electrochromatography: application to the separation of amino acids, ß-blockers, and nucleotides.
Mikrochim Acta
; 186(9): 636, 2019 08 20.
Article
en En
| MEDLINE
| ID: mdl-31432257
ABSTRACT
The preparation of an organic polymer monolithic column modified with an amino acid ionic liquid and graphene oxide (AAIL-GO) and its application to capillary electrochromatography (CEC) was described. The AAIL tetramethylammonium-L-arginine was bonded to a monolithic column that was previously modified with graphene oxide by using an hydrochloride/N-hydroxysuccinimide coupling reaction. The morphology of a poly(glycidyl methacrylate-co-ethylene dimethacrylate) monolith was examined by scanning electron microscopy. The incorporation of AAIL and graphene oxide was detected by infrared spectroscopy and elemental analysis. The resulting monolithic column produced a strong and stable electroosmotic flow from the anode to the cathode in the pH range from 3 to 9. Compared with a column modified with AAIL or graphene oxide only, the AAIL-GO-modified column has a better separation ability for amino acids, ß-blockers, and nucleotides (the resolution of three amino acids 2.231 and 2.036, ß-blockers 2.779 and 2.470 and nucleotides 8.345 and 3.321). Molecular modeling was applied to demonstrate the separation mechanism of small molecules which showed a good support for experimental results. Graphical abstract Schematic representation of capillary electrochromatography (CEC) systems with an amino acid ionic liquid-graphene oxide modified organic polymer monolithic column as stationary phases for separation of amino acids, ß-blockers, and nucleotides.
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1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Polímeros
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Antagonistas Adrenérgicos beta
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Líquidos Iónicos
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Electrocromatografía Capilar
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Aminoácidos
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Grafito
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Nucleótidos
Idioma:
En
Revista:
Mikrochim Acta
Año:
2019
Tipo del documento:
Article