Effective electrochemotherapy with curcumin in MDA-MB-231-human, triple negative breast cancer cells: A global proteomics study.
Bioelectrochemistry
; 131: 107350, 2020 Feb.
Article
en En
| MEDLINE
| ID: mdl-31518962
ABSTRACT
Curcumin (Cur), the yellow pigment of well-known turmeric (Curcuma longa L.) is effective in multiple cancers including triple negative breast cancer (TNBC). In combination with electrical pulses (EP), enhanced effects of curcumin (Curâ¯+â¯EP) are observed in TNBC cells. To gain insights into the mechanisms of enhanced anticancer effects of Curâ¯+â¯EP, we studied the proteins involved in the anticancer activity of Curâ¯+â¯EP in MDA-MB-231, human TNBC cells using high-throughput global proteomics. A curcumin dose of 50⯵M was applied with eight, 1200â¯V/cm, 100⯵s pulses, the most commonly used electrochemotherapy (ECT) parameter in clinics. Results show that the Curâ¯+â¯EP treatment reduced the clonogenic ability in MDA-MB-231 cells, with the induction of apoptosis. Proteomic analysis identified a total of 1456 proteins, of which 453 proteins were differentially regulated, including kinases, heat shock proteins, transcription factors, structural proteins, and metabolic enzymes. Eight key glycolysis proteins (ALDOA, ENO2, LDHA, LDHB, PFKP, PGM1, PGAM1 and PGK1) were downregulated in Curâ¯+â¯EP from Cur. There was a switch in the metabolism with upregulation of 10 oxidative phosphorylation pathway proteins and 8 tricarboxylic acid (TCA) cycle proteins in the Curâ¯+â¯EP sample, compared to curcumin. These results provide novel systematic insights into the mechanisms of ECT with curcumin.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Curcumina
/
Proteómica
/
Electroquimioterapia
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Neoplasias de la Mama Triple Negativas
/
Proteínas de Neoplasias
/
Antineoplásicos
Tipo de estudio:
Prognostic_studies
Límite:
Female
/
Humans
Idioma:
En
Revista:
Bioelectrochemistry
Asunto de la revista:
BIOQUIMICA
Año:
2020
Tipo del documento:
Article
País de afiliación:
Estados Unidos