D-Aspartate oxidase: distribution, functions, properties, and biotechnological applications.
Appl Microbiol Biotechnol
; 104(7): 2883-2895, 2020 Apr.
Article
en En
| MEDLINE
| ID: mdl-32043187
ABSTRACT
Recently, substantial levels of acidic D-amino acids, such as D-aspartate and D-glutamate, have been identified in many organisms, from bacteria to mammals, suggesting that acidic D-amino acids have multiple physiological significances. Although acidic D-amino acids found in animals primarily originate from foodstuffs and/or bacteria, the D-aspartate-synthesizing enzyme aspartate racemase is identified in various animals. In eukaryotic organisms, acidic D-amino acids are primarily degraded by the flavoenzyme D-aspartate oxidase (DDO). DDO is found in multiple eukaryotic organisms and may play important roles in acidic D-amino acid utilization, elimination, and intracellular level regulation. Moreover, owing to its perfect enantioselectivity and stereoselectivity, DDO may be a valuable tool in several biotechnological applications, including the identification and quantification of acidic D-amino acids. In this mini-review, previous DDO reports are summarized and the potential bioengineering and biotechnological applications of DDO are discussed. Key Points ã»Occurrence and distribution ofd-aspartate oxidase. ã»Fundamental properties of d -aspartate oxidase of various eukaryotic organisms. ã»Biotechnological applications and potential engineering ofd-aspartate oxidase.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
D-Aspartato Oxidasa
Límite:
Animals
/
Humans
Idioma:
En
Revista:
Appl Microbiol Biotechnol
Año:
2020
Tipo del documento:
Article
País de afiliación:
Japón