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PACT/PRKRA and p53 regulate transcriptional activity of DMRT1.
Fujitani, Kazuko; Otomo, Asako; Nagayama, Yuto; Tachibana, Taro; Kato, Rika; Kawashima, Yusuke; Kodera, Yoshio; Kato, Tomoko; Takada, Shuji; Tamura, Kei; Takamatsu, Nobuhiko; Ito, Michihiko.
Afiliación
  • Fujitani K; Kitasato University, Gene Analysis Center, School of Medicine, Sagamihara, Japan.
  • Otomo A; Tokai University School of Medicine, Department of Molecular Life Sciences, Isehara, Japan.
  • Nagayama Y; Osaka City University, Department of Bioengineering, Graduate School of Engineering, Osaka, Japan.
  • Tachibana T; Osaka City University, Department of Bioengineering, Graduate School of Engineering, Osaka, Japan.
  • Kato R; Cell Engineering Corporation, Osaka, Japan.
  • Kawashima Y; Kitasato University, Department of Physics, School of Science, Sagamihara, Japan.
  • Kodera Y; Kitasato University, Department of Physics, School of Science, Sagamihara, Japan.
  • Kato T; Kitasato University, Department of Physics, School of Science, Sagamihara, Japan.
  • Takada S; National Research Institute for Child Health and Development, Department of Systems BioMedicine, Tokyo, Japan.
  • Tamura K; National Research Institute for Child Health and Development, Department of Systems BioMedicine, Tokyo, Japan.
  • Takamatsu N; Kitasato University, Department of Bioscience, School of Science, Sagamihara, Japan.
  • Ito M; Kitasato University, Department of Bioscience, School of Science, Sagamihara, Japan.
Genet Mol Biol ; 43(2): e20190017, 2020.
Article en En | MEDLINE | ID: mdl-32251494
The transcription factor DMRT1 (doublesex and mab-3 related transcription factor) has two distinct functions, somatic-cell masculinization and germ-cell development in some vertebrate species, including mouse and the African clawed frog Xenopus laevis. However, its transcriptional regulation remains unclear. We tried to identify DMRT1-interacting proteins from X. laevis testes by immunoprecipitation with an anti-DMRT1 antibody and MS/MS analysis, and selected three proteins, including PACT/PRKRA (Interferon-inducible double-stranded RNA dependent protein kinase activator A) derived from testes. Next, we examined the effects of PACT/PRKRA and/or p53 on the transcriptional activity of DMRT1. In transfected 293T cells, PACT/PRKRA and p53 significantly enhanced and repressed DMRT1-driven luciferase activity, respectively. We also observed that the enhanced activity by PACT/PRKRA was strongly attenuated by p53. Moreover, in situ hybridization analysis of Pact/Prkra mRNA in tadpole gonads indicated high expression in female and male germline stem cells. Taken together, these findings suggest that PACT/PRKRA and p53 might positively and negatively regulate the activity of DMRT1, respectively, for germline stem cell fate.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Genet Mol Biol Año: 2020 Tipo del documento: Article País de afiliación: Japón
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Tipo de estudio: Prognostic_studies Idioma: En Revista: Genet Mol Biol Año: 2020 Tipo del documento: Article País de afiliación: Japón