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Ribosome-associated vesicles: A dynamic subcompartment of the endoplasmic reticulum in secretory cells.
Carter, Stephen D; Hampton, Cheri M; Langlois, Robert; Melero, Roberto; Farino, Zachary J; Calderon, Michael J; Li, Wen; Wallace, Callen T; Tran, Ngoc Han; Grassucci, Robert A; Siegmund, Stephanie E; Pemberton, Joshua; Morgenstern, Travis J; Eisenman, Leanna; Aguilar, Jenny I; Greenberg, Nili L; Levy, Elana S; Yi, Edward; Mitchell, William G; Rice, William J; Wigge, Christoph; Pilli, Jyotsna; George, Emily W; Aslanoglou, Despoina; Courel, Maïté; Freyberg, Robin J; Javitch, Jonathan A; Wills, Zachary P; Area-Gomez, Estela; Shiva, Sruti; Bartolini, Francesca; Volchuk, Allen; Murray, Sandra A; Aridor, Meir; Fish, Kenneth N; Walter, Peter; Balla, Tamas; Fass, Deborah; Wolf, Sharon G; Watkins, Simon C; Carazo, José María; Jensen, Grant J; Frank, Joachim; Freyberg, Zachary.
Afiliación
  • Carter SD; Division of Biology and Biological Engineering, California Institute of Technology, Pasadena, CA 91125, USA.
  • Hampton CM; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
  • Langlois R; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
  • Melero R; Biocomputing Unit, Centro Nacional de Biotecnología-CSIC, Darwin 3, Campus Universidad Autónoma, 28049 Madrid, Spain.
  • Farino ZJ; Department of Psychiatry, University of Pittsburgh, Pittsburgh, PA 15213, USA.
  • Calderon MJ; Department of Cell Biology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
  • Li W; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
  • Wallace CT; Department of Cell Biology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
  • Tran NH; HHMI, Department of Biochemistry and Biophysics, University of California, San Francisco, San Francisco, CA 94143, USA.
  • Grassucci RA; Department of Biochemistry and Molecular Biophysics, Columbia University, New York, NY 10032, USA.
  • Siegmund SE; Department of Cellular, Molecular and Biophysical Studies, Columbia University Medical Center, New York, NY 10032, USA.
  • Pemberton J; Department of Neurology, Columbia University, New York, NY 10032, USA.
  • Morgenstern TJ; Section on Molecular Signal Transduction, Program for Developmental Neuroscience, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, MD 20892, USA.
  • Eisenman L; Department of Psychiatry, Columbia University, New York, NY 10032, USA.
  • Aguilar JI; Division of Molecular Therapeutics, New York State Psychiatric Institute, New York, NY 10032, USA.
  • Greenberg NL; Department of Neurobiology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
  • Levy ES; Department of Psychiatry, Columbia University, New York, NY 10032, USA.
  • Yi E; Division of Molecular Therapeutics, New York State Psychiatric Institute, New York, NY 10032, USA.
  • Mitchell WG; Department of Psychiatry, Columbia University, New York, NY 10032, USA.
  • Rice WJ; Division of Molecular Therapeutics, New York State Psychiatric Institute, New York, NY 10032, USA.
  • Wigge C; Department of Psychiatry, Columbia University, New York, NY 10032, USA.
  • Pilli J; Division of Molecular Therapeutics, New York State Psychiatric Institute, New York, NY 10032, USA.
  • George EW; Department of Psychiatry, Columbia University, New York, NY 10032, USA.
  • Aslanoglou D; Division of Molecular Therapeutics, New York State Psychiatric Institute, New York, NY 10032, USA.
  • Courel M; Department of Psychiatry, Columbia University, New York, NY 10032, USA.
  • Freyberg RJ; Division of Molecular Therapeutics, New York State Psychiatric Institute, New York, NY 10032, USA.
  • Javitch JA; New York Structural Biology Center, New York, NY 10027, USA.
  • Wills ZP; New York Structural Biology Center, New York, NY 10027, USA.
  • Area-Gomez E; Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA 15261, USA.
  • Shiva S; Department of Psychiatry, University of Pittsburgh, Pittsburgh, PA 15213, USA.
  • Bartolini F; Department of Psychiatry, University of Pittsburgh, Pittsburgh, PA 15213, USA.
  • Volchuk A; CNRS-UMR7622, Institut de Biologie Paris-Seine, Université Pierre & Marie Curie, 75252 Paris, France.
  • Murray SA; Department of Psychiatry, University of Pittsburgh, Pittsburgh, PA 15213, USA.
  • Aridor M; Department of Psychiatry, Columbia University, New York, NY 10032, USA.
  • Fish KN; Division of Molecular Therapeutics, New York State Psychiatric Institute, New York, NY 10032, USA.
  • Walter P; Department of Neurobiology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
  • Balla T; Department of Neurology, Columbia University, New York, NY 10032, USA.
  • Fass D; Department of Pharmacology and Chemical Biology, University of Pittsburgh, Pittsburgh, PA 15261, USA.
  • Wolf SG; Vascular Medicine Institute, University of Pittsburgh, Pittsburgh, PA 15261, USA.
  • Watkins SC; Center for Metabolism and Mitochondrial Medicine, University of Pittsburgh, Pittsburgh, PA 15261, USA.
  • Carazo JM; Department of Pathology and Cell Biology, Columbia University, New York, NY 10032, USA.
  • Jensen GJ; Program in Cell Biology, Hospital for Sick Children, Toronto, Ontario, Canada.
  • Frank J; Department of Cell Biology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
  • Freyberg Z; Department of Cell Biology, University of Pittsburgh, Pittsburgh, PA 15213, USA.
Sci Adv ; 6(14): eaay9572, 2020 04.
Article en En | MEDLINE | ID: mdl-32270040
ABSTRACT
The endoplasmic reticulum (ER) is a highly dynamic network of membranes. Here, we combine live-cell microscopy with in situ cryo-electron tomography to directly visualize ER dynamics in several secretory cell types including pancreatic ß-cells and neurons under near-native conditions. Using these imaging approaches, we identify a novel, mobile form of ER, ribosome-associated vesicles (RAVs), found primarily in the cell periphery, which is conserved across different cell types and species. We show that RAVs exist as distinct, highly dynamic structures separate from the intact ER reticular architecture that interact with mitochondria via direct intermembrane contacts. These findings describe a new ER subcompartment within cells.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Ribosomas / Vesículas Citoplasmáticas / Retículo Endoplásmico Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Animals Idioma: En Revista: Sci Adv Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
Imprimir
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PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Ribosomas / Vesículas Citoplasmáticas / Retículo Endoplásmico Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Animals Idioma: En Revista: Sci Adv Año: 2020 Tipo del documento: Article País de afiliación: Estados Unidos