Influence of dietary aconitine and nicotine on the gut microbiota of two lepidopteran herbivores.
Arch Insect Biochem Physiol
; 104(3): e21676, 2020 Jul.
Article
en En
| MEDLINE
| ID: mdl-32323892
ABSTRACT
The gut microbiota plays an important role in pheromone production, pesticide degradation, vitamin synthesis, and pathogen prevention in the host animal. Therefore, similar to gut morphology and digestive enzyme activity, the gut microbiota may also get altered under plant defensive compound-induced stress. To test this hypothesis, Dendrolimus superans larvae were fed either aconitine- or nicotine-treated fresh leaves of Larix gmelinii, and Lymantria dispar larvae were fed either aconitine- or nicotine-treated fresh leaves of Salix matsudana. Subsequently, the larvae were sampled 72hr after diet administration and DNA extracted from larval enteric canals were employed for gut microbial 16S ribosomal RNA gene sequencing (338 F and 806 R primers). The sequence analysis revealed that dietary nicotine and aconitine influenced the dominant bacteria in the larval gut and determined their abundance. Moreover, the effect of either aconitine or nicotine on D. superans and L. dispar larvae had a greater dependence on insect species than on secondary plant metabolites. These findings further our understanding of the interaction between herbivores and host plants and the coevolution of plants and insects.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Aconitina
/
Microbioma Gastrointestinal
/
Mariposas Nocturnas
/
Nicotina
Límite:
Animals
Idioma:
En
Revista:
Arch Insect Biochem Physiol
Asunto de la revista:
BIOLOGIA
/
BIOQUIMICA
Año:
2020
Tipo del documento:
Article
País de afiliación:
China