An immuno-dot blot assay for screening histidine kinase inhibitors.
Anal Biochem
; 600: 113765, 2020 07 01.
Article
en En
| MEDLINE
| ID: mdl-32360198
ABSTRACT
Two-component signal transduction systems (TCSs), consisting of a histidine kinase (HK) and its cognate response regulator, are ubiquitous among bacteria and are associated with the virulence of pathogens. TCSs are potential targets for alternative antibiotics and antivirulence agents. It is, thus, very important to determine HK activity in bacterial TCSs. Here, we describe an immuno-dot blot assay for the inhibition profiling of HKs using the anti-N3-phosphohistidine antibody. This simple method promises reliable detection of HK activity, and it is likely applicable in high-throughput screening of HK inhibitors.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Quinonas
/
Inhibidores de Proteínas Quinasas
/
Histidina Quinasa
Tipo de estudio:
Diagnostic_studies
/
Screening_studies
Idioma:
En
Revista:
Anal Biochem
Año:
2020
Tipo del documento:
Article
País de afiliación:
Japón