Novel bacterial clade reveals origin of form I Rubisco.
Nat Plants
; 6(9): 1158-1166, 2020 09.
Article
en En
| MEDLINE
| ID: mdl-32868887
ABSTRACT
Rubisco sustains the biosphere through the fixation of CO2 into biomass. In plants and cyanobacteria, form I Rubisco is structurally comprised of large and small subunits, whereas all other Rubisco forms lack small subunits. The rise of the form I complex through the innovation of small subunits represents a key, yet poorly understood, transition in Rubisco's evolution. Through metagenomic analyses, we discovered a previously uncharacterized clade sister to form I Rubisco that evolved without small subunits. This clade diverged before the evolution of cyanobacteria and the origin of the small subunit; thus, it provides a unique reference point to advance our understanding of form I Rubisco evolution. Structural and kinetic data presented here reveal how a proto-form I Rubisco assembled and functioned without the structural stability imparted from small subunits. Our findings provide insight into a key evolutionary transition of the most abundant enzyme on Earth and the predominant entry point for nearly all global organic carbon.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fotosíntesis
/
Ribulosa-Bifosfato Carboxilasa
/
Estructura Molecular
/
Cianobacterias
/
Fenómenos Fisiológicos de las Plantas
Idioma:
En
Revista:
Nat Plants
Año:
2020
Tipo del documento:
Article
País de afiliación:
Estados Unidos