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Hypoxia-inducible factor-1alpha and nitric oxide synthases in bovine follicles close to ovulation and early luteal angiogenesis.
Berisha, Bajram; Schams, Dieter; Sinowatz, Fred; Rodler, Daniela; Pfaffl, Michael W.
Afiliación
  • Berisha B; Animal Biotechnology, Faculty of Agriculture and Veterinary, University of Prishtina, Prishtinë, Kosovo.
  • Schams D; Animal Physiology and Immunology Weihenstephan, Technical University of Munich, Freising, Germany.
  • Sinowatz F; Animal Physiology and Immunology Weihenstephan, Technical University of Munich, Freising, Germany.
  • Rodler D; Department of Veterinary Sciences, Ludwig Maximilian University of Munich, Munich, Germany.
  • Pfaffl MW; Department of Veterinary Sciences, Ludwig Maximilian University of Munich, Munich, Germany.
Reprod Domest Anim ; 55(11): 1573-1584, 2020 Nov.
Article en En | MEDLINE | ID: mdl-32869370
ABSTRACT
The objective of the study was to characterize expression patterns of hypoxia-inducible factor-1alpha (HIF1A), inducible nitric oxide synthase (iNOS) and endothelial (eNOS) isoforms in time-defined follicle classes before and after GnRH application in the cow. Ovaries containing pre-ovulatory follicles or corpora lutea were collected by transvaginal ovariectomy (n = 5 cows/group) as follow (I) before GnRH administration; (II) 4h after GnRH; (III) 10h after GnRH; (IV) 20h after GnRH; (V) 25h after GnRH; and (VI) 60h after GnRH (early corpus luteum). The mRNA abundance of HIF1A in the follicle group before GnRH was high, followed by a significant down regulation afterwards with a minimum level 25h after GnRH (close to ovulation) and significant increase only after ovulation. The mRNA abundance of iNOS before GnRH was high, decreased significantly during LH surge, with minimum levels afterwards. In contrast, the mRNA of eNOS decreased in the follicle group 20h after GnRH, followed by a rapid and significant upregulation just after ovulation. Immunohistochemically, the granulosa cells of antral follicles and the eosinophils of the theca tissue as well of the early corpus luteum showed a strong staining for HIF1A. The location of the eosinophils could be clearly demonstrated by immunostaining with an eosinophil-specific antibody (EMBP) and transmission electron microscopy. In conclusion, the parallel and acute regulated expression patterns of HIF1A and NOS isoforms, specifically during the interval between the LH surge and ovulation, indicate that these paracrine factors are involved in the local mechanisms, regulating final follicle maturation, ovulation and early luteal angiogenesis.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Ovulación / Bovinos / Subunidad alfa del Factor 1 Inducible por Hipoxia / Folículo Ovárico Límite: Animals Idioma: En Revista: Reprod Domest Anim Asunto de la revista: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Año: 2020 Tipo del documento: Article

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Ovulación / Bovinos / Subunidad alfa del Factor 1 Inducible por Hipoxia / Folículo Ovárico Límite: Animals Idioma: En Revista: Reprod Domest Anim Asunto de la revista: MEDICINA REPRODUTIVA / MEDICINA VETERINARIA Año: 2020 Tipo del documento: Article