Transformation of yeast spheroplasts without cell fusion.
Anal Biochem
; 163(2): 391-7, 1987 Jun.
Article
en En
| MEDLINE
| ID: mdl-3310730
ABSTRACT
The efficiency of genetic transformation of Saccharomyces cerevisiae spheroplasts has been increased 10- to 100-fold over previously published procedures. Optimal transformation frequencies for single-stranded and double-stranded replicating plasmids are 2 X 10(7) and 5 X 10(6) transformants/microgram, respectively. At saturating DNA concentrations, 12 and 3%, respectively, of the viable spheroplasts contain plasmid DNA. The percentage of transformants that have undergone nuclear fusion varies from 0.1 to 3%, indicating that fusion is not required for the uptake of DNA by yeast spheroplasts.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Saccharomyces cerevisiae
/
Transformación Genética
Idioma:
En
Revista:
Anal Biochem
Año:
1987
Tipo del documento:
Article