Pinellia ternata attenuates carotid artery intimal hyperplasia and increases endothelial progenitor cell activity via the PI3K/Akt signalling pathway in wire-injured rats.
Pharm Biol
; 58(1): 1184-1191, 2020 Dec.
Article
en En
| MEDLINE
| ID: mdl-33253601
ABSTRACT
CONTEXT Clinically, Pinellia ternata (Thunb.) Breit. (Araceae) (PT) has been widely used in the treatment of atherosclerosis and hyperlipidaemia, but the underlying mechanisms are still not clearly understood. OBJECTIVE:
This research was conducted to confirm the mechanism by which PT affects carotid artery intimal hyperplasia. MATERIALS ANDMETHODS:
An intestinal hyperplasia Sprague-Dawley rat model was established by carotid artery injury. The rats were randomly divided into five groups (n = 8) sham, model, PT (with daily intragastric administration of 10 g/mL/kg PT tubers water extract), PT+LY294002 (with intraperitoneal injection of 50 mg/kg LY294002 + 10 g/mL/kg PT) and endothelial progenitor cells (EPCs) (with injection of 5 × 105/cells), and treated for 4 or 8 weeks.RESULTS:
HE staining showed that PT attenuated intimal hyperplasia. RT-PCR, Western blotting and immunohistochemistry showed that PT increased the expression of vascular endothelial growth factor (VEGF) and eNOS in the atherosclerotic carotid artery. PT increased the Dil-acLDL+/FITC-UEA-1+ population (from 0.41 ± 0.085% to 0.60 ± 0.092%) in the blood, decreased TCHO, TG, LDL-C, IL-6 and TNF-α levels, and increased HDL-C and IL-10 levels in the blood. However, these changes were reversed by the PI3K/Akt pathway inhibitor LY294002. DISCUSSION ANDCONCLUSIONS:
PT can be developed as an atherosclerosis and carotid intimal hyperplasia treatment drug. Therefore, further study will focus on the effects of PT on intimal hyperplasia in wire-injured atherosclerosis patients and explore in depth some other relevant molecular mechanisms.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Extractos Vegetales
/
Transducción de Señal
/
Túnica Íntima
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Fosfatidilinositol 3-Quinasas
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Traumatismos de las Arterias Carótidas
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Pinellia
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Proteína Oncogénica v-akt
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Células Progenitoras Endoteliales
Tipo de estudio:
Prognostic_studies
/
Qualitative_research
Límite:
Animals
Idioma:
En
Revista:
Pharm Biol
Año:
2020
Tipo del documento:
Article
País de afiliación:
China