A Hitchhiker's Guide to Click-Chemistry with Nucleic Acids.
Chem Rev
; 121(12): 7122-7154, 2021 06 23.
Article
en En
| MEDLINE
| ID: mdl-33443411
ABSTRACT
Click chemistry is an immensely powerful technique for the fast and efficient covalent conjugation of molecular entities. Its broad scope has positively impacted on multiple scientific disciplines, and its implementation within the nucleic acid field has enabled researchers to generate a wide variety of tools with application in biology, biochemistry, and biotechnology. Azide-alkyne cycloadditions (AAC) are still the leading technology among click reactions due to the facile modification and incorporation of azide and alkyne groups within biological scaffolds. Application of AAC chemistry to nucleic acids allows labeling, ligation, and cyclization of oligonucleotides efficiently and cost-effectively relative to previously used chemical and enzymatic techniques. In this review, we provide a guide to inexperienced and knowledgeable researchers approaching the field of click chemistry with nucleic acids. We discuss in detail the chemistry, the available modified-nucleosides, and applications of AAC reactions in nucleic acid chemistry and provide a critical view of the advantages, limitations, and open-questions within the field.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Azidas
/
Ácidos Nucleicos
/
Alquinos
/
Química Clic
Idioma:
En
Revista:
Chem Rev
Año:
2021
Tipo del documento:
Article
País de afiliación:
Reino Unido