Serum proteomes of Santa Gertrudis cattle before and after infestation with Rhipicephalus australis ticks.
Parasite Immunol
; 43(7): e12836, 2021 07.
Article
en En
| MEDLINE
| ID: mdl-33843060
ABSTRACT
Previous studies have applied genomics and transcriptomics to identify immune and genetic markers as key indicator traits for cattle tick susceptibility/resistance; however, results differed between breeds, and there is lack of information on the use of host proteomics. Serum samples from Santa Gertrudis cattle (naïve and phenotyped over 105 days as tick-resistant [TR] or tick-susceptible [TS]) were used to conduct differential abundance analyses of protein profiles. Serum proteins were digested into peptides followed by identification and quantification using sequential window acquisition of all instances of theoretical fragment ion mass spectrometry. Before tick infestation, abundance of 28 proteins differed significantly (adjusted P < 10-5 ) between TR and TS. These differences were also observed following tick infestation (TR vs TS) with a further eight differentially abundant proteins in TR cattle, suggesting possible roles in adaptive responses. The intragroup comparisons (TS-0 vs TS and TR-0 vs TR) showed that tick infestation elicited quite similar responses in both groups of cattle, but with relatively stronger responses in TR cattle. Many of the significantly differentially abundant proteins in TR Santa Gertrudis cattle (before and after tick infestation) were associated with immune responses including complement factors, chemotaxis for immune cells and acute-phase responses.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Infestaciones por Garrapatas
/
Enfermedades de los Bovinos
/
Rhipicephalus
Tipo de estudio:
Prognostic_studies
Límite:
Animals
Idioma:
En
Revista:
Parasite Immunol
Año:
2021
Tipo del documento:
Article
País de afiliación:
Australia