Your browser doesn't support javascript.
loading
Cartiotonic steroids affect monolayer permeability in lymphatic endothelial cells.
Horvat, Darijana; Afroze, Syeda H; Cromer, Walter E; Pantho, Ahmed F; Ashraf, A H M Zuberi; Kuehl, Thomas J; Zawieja, David C; Uddin, Mohammad Nasir.
Afiliación
  • Horvat D; Orion Institute for Translational Medicine, Temple, TX, USA.
  • Afroze SH; Emergent Biotechnologies LLC, Temple, TX, USA.
  • Cromer WE; Orion Institute for Translational Medicine, Temple, TX, USA.
  • Pantho AF; Emergent Biotechnologies LLC, Temple, TX, USA.
  • Ashraf AHMZ; Department of Medical Physiology, Texas A&M Health Science Center College of Medicine, Bryan, TX, USA.
  • Kuehl TJ; Orion Institute for Translational Medicine, Temple, TX, USA.
  • Zawieja DC; Emergent Biotechnologies LLC, Temple, TX, USA.
  • Uddin MN; Baylor Scott and White Health Medical Center, Temple, TX, USA.
Mol Cell Biochem ; 476(8): 3207-3213, 2021 Aug.
Article en En | MEDLINE | ID: mdl-33866492
Edema is common in preeclampsia (preE), a hypertensive disorder of pregnancy. Cardiotonic steroids (CTSs) such as marinobufagenin (MBG) are involved in the pathogenesis of preE. To assess whether CTSs are involved in the leakage of lymphatic endothelial cell (LEC), we evaluated their effect on monolayer permeability of LECs (MPLEC) in culture. A rat mesenteric LECs were treated with DMSO (vehicle), and CTSs (MBG, CINO, OUB) at concentrations of 1, 10, and 100 nM. Some LECs were pretreated with 1 µM L-NAME (N-Nitro-L-Arginine Methyl Ester) before adding 100 nM MBG or cinobufotalin (CINO). Expression of ß-catenin and vascular endothelial (VE)-cadherin in CTS-treated LECs was measured by immunofluorescence and MPLEC was quantified using a fluorescence plate reader. Western blot was performed to measure ß-catenin and VE-cadherin protein levels and myosin light chain 20 (MLC20) phosphorylation. MBG (≥ 1 nM) and CINO (≥ 10 nM) caused an increase (p < 0.05) in the MPLEC compared to DMSO while ouabain (OUB) had no effect. Pretreatment of LECs with 1 µM L-NAME attenuated (p < 0.05) the MPLEC. The ß-catenin expression in LECs was downregulated (p < 0.05) by MBG and CINO. However, there was no effect on the LECs tight junctions for the CINO group. VE-cadherin expression was downregulated (p < 0.05) by CINO, and MLC20 phosphorylation was upregulated (p < 0.05) by MBG. We demonstrated that MBG and CINO caused an increase in the MPLEC, which were attenuated by L-NAME pretreatment. The data suggest that CTSs exert their effect via nitric-oxide-dependent signaling pathway and may be involved in vascular leak syndrome of LEC lining in preE.
Asunto(s)
Palabras clave

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Vasoconstrictores / Bufanólidos / Permeabilidad de la Membrana Celular / Regulación de la Expresión Génica / Células Endoteliales / Óxido Nítrico Límite: Animals Idioma: En Revista: Mol Cell Biochem Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Vasoconstrictores / Bufanólidos / Permeabilidad de la Membrana Celular / Regulación de la Expresión Génica / Células Endoteliales / Óxido Nítrico Límite: Animals Idioma: En Revista: Mol Cell Biochem Año: 2021 Tipo del documento: Article País de afiliación: Estados Unidos