Detection and Identification of Wolbachia pipientis Strains in Mosquito Eggs Using Attenuated Total Reflection Fourier Transform Infrared (ATR FT-IR) Spectroscopy.
Appl Spectrosc
; 75(8): 1003-1011, 2021 Aug.
Article
en En
| MEDLINE
| ID: mdl-34110943
ABSTRACT
The global fight against mosquito-borne viral diseases has in recent years been bolstered by the introduction of the endosymbiotic bacteria Wolbachia to vector populations, which in host mosquitoes suppresses the transmissibility of several viruses. Researchers engaged on this front of the battle often need to know the Wolbachia infection status of individual mosquitoes, as the intervention progresses and the mosquitoes become established in the target population. Previously, we successfully applied attenuated total reflection Fourier transform infrared spectroscopy to the detection of Wolbachia in adult Aedes aegypti mosquitoes; here we apply the same principles to Aedes eggs, with sensitivity and selectivity > 0.95. Further, we successfully distinguish between infections in eggs of the wMel and wMelPop strains of Wolbachia pipientis, with a classification error of 3%. The disruption of host lipid profile by Wolbachia is found to be a key driver in spectral differences between these sample classes.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Aedes
/
Wolbachia
Tipo de estudio:
Diagnostic_studies
Límite:
Animals
Idioma:
En
Revista:
Appl Spectrosc
Año:
2021
Tipo del documento:
Article
País de afiliación:
Australia