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New Red-Emitting Chloride-Sensitive Fluorescent Protein with Biological Uses.
Salto, Rafael; Giron, Maria D; Puente-Muñoz, Virginia; Vilchez, Jose D; Espinar-Barranco, Laura; Valverde-Pozo, Javier; Arosio, Daniele; Paredes, Jose M.
Afiliación
  • Salto R; Department of Biochemistry and Molecular Biology II, Faculty of Pharmacy, Unidad de Excelencia en Quimica Aplicada a Biomedicina y Medioambiente (UEQ), University of Granada, Cartuja Campus, 18071 Granada, Spain.
  • Giron MD; Department of Biochemistry and Molecular Biology II, Faculty of Pharmacy, Unidad de Excelencia en Quimica Aplicada a Biomedicina y Medioambiente (UEQ), University of Granada, Cartuja Campus, 18071 Granada, Spain.
  • Puente-Muñoz V; Department of Physical Chemistry, Faculty of Pharmacy, Unidad de Excelencia en Quimica Aplicada a Biomedicina y Medioambiente (UEQ), University of Granada, C. U. Cartuja, 18071 Granada, Spain.
  • Vilchez JD; Department of Biochemistry and Molecular Biology II, Faculty of Pharmacy, Unidad de Excelencia en Quimica Aplicada a Biomedicina y Medioambiente (UEQ), University of Granada, Cartuja Campus, 18071 Granada, Spain.
  • Espinar-Barranco L; Department of Physical Chemistry, Faculty of Pharmacy, Unidad de Excelencia en Quimica Aplicada a Biomedicina y Medioambiente (UEQ), University of Granada, C. U. Cartuja, 18071 Granada, Spain.
  • Valverde-Pozo J; Department of Physical Chemistry, Faculty of Pharmacy, Unidad de Excelencia en Quimica Aplicada a Biomedicina y Medioambiente (UEQ), University of Granada, C. U. Cartuja, 18071 Granada, Spain.
  • Arosio D; Consiglio Nazionale delle Ricerche (CNR), Istituto di Biofisica (IBF-CNR), 38123 Trento, Italy.
  • Paredes JM; Department of Physical Chemistry, Faculty of Pharmacy, Unidad de Excelencia en Quimica Aplicada a Biomedicina y Medioambiente (UEQ), University of Granada, C. U. Cartuja, 18071 Granada, Spain.
ACS Sens ; 6(7): 2563-2573, 2021 07 23.
Article en En | MEDLINE | ID: mdl-34148347
ABSTRACT
A new chloride-sensitive red fluorescent protein derived from Entacmaea quadricolor is described. We found that mBeRFP exhibited moderate sensitivity to chloride and, via site-directed mutagenesis (S94V and R205Y), we increased the chloride affinity by more than an order of magnitude (kd = 106 ± 6 mM) at physiological pH. In addition, cis-trans isomerization of the chromophore produces a dual emission band with different chloride sensitivities, which allowed us to develop a ratiometric methodology to measure intracellular chloride concentrations.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Cloruros Tipo de estudio: Diagnostic_studies Idioma: En Revista: ACS Sens Año: 2021 Tipo del documento: Article País de afiliación: España
Texto completo
Imprimir
XML
PubMed Links
Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Cloruros Tipo de estudio: Diagnostic_studies Idioma: En Revista: ACS Sens Año: 2021 Tipo del documento: Article País de afiliación: España