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Autologous bone marrow-derived mesenchymal stem cells provide complete regeneration in a rabbit model of the Achilles tendon bundle rupture.
Chailakhyan, Ruben K; Kon, Elizaveta; Shekhter, Anatoly B; Ivannikov, Sergey V; Telpukhov, Vladimir I; Grosheva, Alla G; Suslin, Dmitry S; Vorobieva, Nataliya N; Gerasimov, Yury V; Churbanov, Semyon N; Kotova, Svetlana; Fayzullin, Alexey L; Lychagin, Alexey V; Lipina, Marina M; Timashev, Peter S.
Afiliación
  • Chailakhyan RK; N.F.Gamaleya National Research Center for Epidemiology & Microbiology of the Ministry of Health of the Russian Federation, 18 Gamaleya St, Moscow, 123098, Russia.
  • Kon E; Institute of Photonic Technologies, Federal Scientific Research Centre "Crystallography and Photonics" of the Russian Academy of Sciences, 2 Pionerskaya St., Moscow, Troitsk, 108840, Russia.
  • Shekhter AB; Department of Traumatology, Orthopedics and Disaster Surgery, Sechenov First Moscow State Medical University (Sechenov University), 2-4 Bolshaya Pirogovskaya St, 119991, Moscow, Russia. elizaveta.kon@humanitas.it.
  • Ivannikov SV; Department of Biomedical Sciences, Humanitas University, Via Rita Levi Montalcini 4, , Milan, Pieve Emanuele, Italy. elizaveta.kon@humanitas.it.
  • Telpukhov VI; Humanitas Clinical and Research Center - IRCCS, via Manzoni 56, 20089, Milan, Rozzano, Italy. elizaveta.kon@humanitas.it.
  • Grosheva AG; Laboratory of Experimental Morphology, Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya St, Moscow, 119991, Russia.
  • Suslin DS; Department of Traumatology, Orthopedics and Disaster Surgery, Sechenov First Moscow State Medical University (Sechenov University), 2-4 Bolshaya Pirogovskaya St, 119991, Moscow, Russia.
  • Vorobieva NN; Laboratory of Regeneration of Skeletal Tissues, Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya St, Moscow, 119991, Russia.
  • Gerasimov YV; Department of Topographical Anatomy and Operative Surgery, Sechenov First Moscow State Medical University (Sechenov University), 15\13 Rossolimo St, Moscow, 119991, Russia.
  • Churbanov SN; N.F.Gamaleya National Research Center for Epidemiology & Microbiology of the Ministry of Health of the Russian Federation, 18 Gamaleya St, Moscow, 123098, Russia.
  • Kotova S; Traumatology Department, Clinical and Diagnostic Center "Medsi, Gruzinskiy Per., 3 A. , Moscow, 123056, Russia.
  • Fayzullin AL; Institute of Photonic Technologies, Federal Scientific Research Centre "Crystallography and Photonics" of the Russian Academy of Sciences, 2 Pionerskaya St., Moscow, Troitsk, 108840, Russia.
  • Lychagin AV; N.F.Gamaleya National Research Center for Epidemiology & Microbiology of the Ministry of Health of the Russian Federation, 18 Gamaleya St, Moscow, 123098, Russia.
  • Lipina MM; Institute of Photonic Technologies, Federal Scientific Research Centre "Crystallography and Photonics" of the Russian Academy of Sciences, 2 Pionerskaya St., Moscow, Troitsk, 108840, Russia.
  • Timashev PS; Laboratory of Advanced Biomaterials, Institute for Regenerative Medicine, Sechenov First Moscow State Medical University (Sechenov University), 8-2 Trubetskaya St, Moscow, 119991, Russia.
Int Orthop ; 45(12): 3263-3276, 2021 12.
Article en En | MEDLINE | ID: mdl-34510279
PURPOSE: To ascertain the role of autologous bone marrow-derived mesenchymal stem cells (BM-MSCs) in the tendon regeneration. METHODS: The study was conducted on 58 Achilles tendons from 29 laboratory Chinchilla adult rabbits. The central bundles of 48 tendons were partially removed and substituted with a tissue-engineered construct consisting of a collagen sponge either loaded with BM-MSCs (n = 24) or cell free (n = 24), placed inside a Vicryl mesh tube. The ends of the resected tendon were inserted in the construct to reach a direct contact with the sponge and sutured to the tube. The animals were sacrificed three and six months post-surgery. Ten intact tendons from five rabbits were used as an untreated control. The tissue samples (n = 30) were stained with haematoxylin and eosin, Picrosirius red, primary antibodies to collagen types I and III and studied by bright-field, phase-contrast, polarized light, and scanning electron microscopies followed by semi-quantitative morphometry. RESULTS: Six months results of cell-loaded scaffolds demonstrated parallel collagen fibres, spindle-shaped tenocytes, and neoangiogenesis. In the control cell-free group, the injured areas were filled with a nonspecific fibrotic tissue with minor foci of incomplete regeneration. The biomechanical tests of 28 tendons taken from 14 rabbits showed that the stiffness of the cell-based reconstructed tendons increased to 98% of the value for the intact samples. CONCLUSION: The obtained results support the hypothesis that the application of BM-MSCs in a tissue-engineered tendon construct leads to the restitution of the tendon tissue.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Tendón Calcáneo / Traumatismos de los Tendones / Células Madre Mesenquimatosas Límite: Animals Idioma: En Revista: Int Orthop Año: 2021 Tipo del documento: Article País de afiliación: Rusia

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Tendón Calcáneo / Traumatismos de los Tendones / Células Madre Mesenquimatosas Límite: Animals Idioma: En Revista: Int Orthop Año: 2021 Tipo del documento: Article País de afiliación: Rusia