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Pneumocystis jirovecii genetic diversity in a Spanish tertiary hospital.
Goterris, Lidia; Pasic, Lana; Guerrero Murillo, Mercedes; Kan, Alex; Anton, Andres; Aguilar Company, Juan; Ruiz-Camps, Isabel; Meyer, Wieland; Martin-Gomez, María Teresa.
Afiliación
  • Goterris L; Microbiology Department, Hospital Universitari Vall d'Hebron, 08035 Barcelona, Spain.
  • Pasic L; Genetics and Microbiology Department, Universitat Autònoma de Barcelona (UAB), 08193 Barcelona, Spain.
  • Guerrero Murillo M; Molecular Mycology Research Laboratory, Centre for Infectious Diseases and Microbiology, Westmead Clinical School, Sydney Medical School, Faculty of Medicine and Health, The University of Sydney, NSW 2050 Sydney, Australia.
  • Kan A; Marie Bashir Institute for Infectious Diseases and Biosecurity, The University of Sydney, NSW 2145 Sydney, Australia.
  • Anton A; Westmead Institute for Medical Research, NSW 2145 Westmead, Australia.
  • Aguilar Company J; Microbiology Department, Hospital Universitari Vall d'Hebron, 08035 Barcelona, Spain.
  • Ruiz-Camps I; Molecular Mycology Research Laboratory, Centre for Infectious Diseases and Microbiology, Westmead Clinical School, Sydney Medical School, Faculty of Medicine and Health, The University of Sydney, NSW 2050 Sydney, Australia.
  • Meyer W; Marie Bashir Institute for Infectious Diseases and Biosecurity, The University of Sydney, NSW 2145 Sydney, Australia.
  • Martin-Gomez MT; Westmead Institute for Medical Research, NSW 2145 Westmead, Australia.
Med Mycol ; 60(1)2021 Dec 08.
Article en En | MEDLINE | ID: mdl-34698858
ABSTRACT
Pneumocystis jirovecii is associated with non-noxious colonization or severe pneumonia in immunocompromised hosts. Epidemiological investigations have been hampered by the lack of a standardized typing scheme. Thus, only partial molecular data on Spanish P. jirovecii cases are available. Recently, a new ISHAM consensus multilocus sequence typing scheme (MLST) targeting ß-TUB, mt26S, CYB, and SOD with a publicly accessible database has been launched to overcome this problem. The molecular epidemiology of P. jirovecii from immunocompromised patients either colonized (n = 50) or having pneumonia (n = 36) seen between 2014 and 2018 at a single center in Barcelona, Spain, was studied. The new ISHAM consensus MSLT scheme was used to investigate the local epidemiology and identify possible unnoticed outbreaks. Mutations in the DHPS gene, not included in the scheme but giving information about potential sulfa treatment failure, were also studied. The study assigned 32 sequence types (ST) to 72.2% pneumonia and 56% colonization cases. The most frequent STs were ST21 (18.5%), ST22 (14.8%), and ST37(14.8%). For non-unique STs, ST3, ST30 and ST31 were found only in pneumonia cases, whereas ST27 was associated exclusively to colonizations. Despite 38 patients sharing similar STs, only two were involved in a potential cross transmission event. No DHPS mutations were identified. The new consensus typing scheme was useful to ascertain the molecular epidemiology of P. jirovecii in our center revealing a high genetic diversity and the potential association of specific STs to colonization and pneumonia cases. LAY

SUMMARY:

A newly described MLST scheme aims at providing a standardized tool to study and compare Pneumocystis jirovecii epidemiology. A high diversity among P. jirovecii isolates from patients in Barcelona, Spain, and a potential association between specific STs and infection/colonization were identified.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Neumonía por Pneumocystis / Pneumocystis carinii Límite: Animals Idioma: En Revista: Med Mycol Asunto de la revista: MEDICINA VETERINARIA / MICROBIOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: España

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Neumonía por Pneumocystis / Pneumocystis carinii Límite: Animals Idioma: En Revista: Med Mycol Asunto de la revista: MEDICINA VETERINARIA / MICROBIOLOGIA Año: 2021 Tipo del documento: Article País de afiliación: España