Rapid, efficient and activation-neutral gene editing of polyclonal primary human resting CD4<sup>+</sup> T cells allows complex functional analyses.

Albanese, Manuel; Ruhle, Adrian; Mittermaier, Jennifer; Mejías-Pérez, Ernesto; Gapp, Madeleine; Linder, Andreas; Schmacke, Niklas A; Hofmann, Katharina; Hennrich, Alexandru A; Levy, David N; Humpe, Andreas; Conzelmann, Karl-Klaus; Hornung, Veit; Fackler, Oliver T; Keppler, Oliver T

Rapid, efficient and activation-neutral gene editing of polyclonal primary human resting CD4⁺ T cells allows complex functional analyses.

Albanese, Manuel; Ruhle, Adrian; Mittermaier, Jennifer; Mejías-Pérez, Ernesto; Gapp, Madeleine; Linder, Andreas; Schmacke, Niklas A; Hofmann, Katharina; Hennrich, Alexandru A; Levy, David N; Humpe, Andreas; Conzelmann, Karl-Klaus; Hornung, Veit; Fackler, Oliver T; Keppler, Oliver T.

Afiliación

Albanese M; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU München, Munich, Germany. Albanese@mvp.uni-muenchen.de.
Ruhle A; Gene Center and Department of Biochemistry, LMU München, Munich, Germany. Albanese@mvp.uni-muenchen.de.
Mittermaier J; Istituto Nazionale di Genetica Molecolare, INGM, "Romeo ed Enrica Invernizzi", Milan, Italy. Albanese@mvp.uni-muenchen.de.
Mejías-Pérez E; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU München, Munich, Germany.
Gapp M; Gene Center and Department of Biochemistry, LMU München, Munich, Germany.
Linder A; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU München, Munich, Germany.
Schmacke NA; Gene Center and Department of Biochemistry, LMU München, Munich, Germany.
Hofmann K; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU München, Munich, Germany.
Hennrich AA; Gene Center and Department of Biochemistry, LMU München, Munich, Germany.
Levy DN; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU München, Munich, Germany.
Humpe A; Gene Center and Department of Biochemistry, LMU München, Munich, Germany.
Conzelmann KK; Gene Center and Department of Biochemistry, LMU München, Munich, Germany.
Hornung V; Department of Medicine II, University Hospital, LMU München, Munich, Germany.
Fackler OT; Gene Center and Department of Biochemistry, LMU München, Munich, Germany.
Keppler OT; Max von Pettenkofer Institute and Gene Center, Virology, National Reference Center for Retroviruses, Faculty of Medicine, LMU München, Munich, Germany.

Nat Methods ; 19(1): 81-89, 2022 01.

Article en En | MEDLINE | ID: mdl-34949807

Asunto(s)

Linfocitos T CD4-Positivos/fisiología; Sistemas CRISPR-Cas/genética; Edición Génica/métodos; Infecciones por VIH/genética; Linfocitos T CD4-Positivos/citología; Linfocitos T CD4-Positivos/virología; Proteína 9 Asociada a CRISPR/genética; Movimiento Celular/genética; Células Cultivadas; ADN; Técnicas de Inactivación de Genes; Infecciones por VIH/metabolismo; Humanos; Glicoproteínas de Membrana/genética; Glicoproteínas de Membrana/metabolismo; Proteínas de Resistencia a Mixovirus/genética; Proteínas de Resistencia a Mixovirus/metabolismo; ARN Guía de Kinetoplastida; Proteína 1 que Contiene Dominios SAM y HD/genética; Transgenes; Factores de Escisión y Poliadenilación de ARNm/genética; Factores de Escisión y Poliadenilación de ARNm/metabolismo

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Linfocitos T CD4-Positivos / Infecciones por VIH / Sistemas CRISPR-Cas / Edición Génica Límite: Humans Idioma: En Revista: Nat Methods Asunto de la revista: TECNICAS E PROCEDIMENTOS DE LABORATORIO Año: 2022 Tipo del documento: Article País de afiliación: Alemania

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