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A Comparative Study for Measuring Serum Ferritin Levels with Three Different Laboratory Methods: Enzyme-Linked Immunosorbent Assay versus Cobas e411 and Cobas Integra 400 Methods.
Dahman, Lotfi S Bin; Sumaily, Khalid M; Sabi, Essa M; Hassan, Mohammed A; Thalab, Abeer M Bin; Sayad, Asrar S; Kolaib, Saleh M Bin; Alhadhrmi, Fatima M.
Afiliación
  • Dahman LSB; Department of Medical Biochemistry, College of Medicine, Hadhramout University, Mukalla 50511, Yemen.
  • Sumaily KM; Clinical Biochemistry Unit, Pathology Department, College of Medicine, King Saud University, Riyadh 11461, Saudi Arabia.
  • Sabi EM; Clinical Biochemistry Unit, Pathology Department, College of Medicine, King Saud University, Riyadh 11461, Saudi Arabia.
  • Hassan MA; Department of Medical Basic Sciences, College of Medicine, Hadhramout University, Mukalla 50511, Yemen.
  • Thalab AMB; Department of Pathology, College of Medicine, Hadhramout University, Mukalla 50511, Yemen.
  • Sayad AS; Department of Obstetrics and Gynecology, College of Medicine, Hadhramout University, Mukalla 50511, Yemen.
  • Kolaib SMB; BSc Candidate of Laboratory Medicine, College of Medicine, Hadhramout University, Mukalla 50511, Yemen.
  • Alhadhrmi FM; BSc Candidate of Laboratory Medicine, College of Medicine, Hadhramout University, Mukalla 50511, Yemen.
Diagnostics (Basel) ; 12(2)2022 Jan 27.
Article en En | MEDLINE | ID: mdl-35204412
ABSTRACT
Different laboratory methods are used to measure serum ferritin levels as a marker of iron status in the general population. This study aimed to compare serum ferritin levels using enzyme-linked immunosorbent assay (ELISA) versus immunochemiluminescence (Cobas e411) and immunoturbidimetric (Cobas Integra 400) methods in terms of sensitivity, specificity and accuracy, and whether they can be used interchangeably. A comparative cross-sectional study enrolled one hundred and six adult Yemeni patients (33 males and 73 females) aged 18-55 years, recruited from the dermatology and cosmetic center of Hadhramout Modern Hospital, Mukalla, Yemen. Serum ferritin levels were measured using ELISA, Cobas e411, and Cobas Integra 400 methods. For method comparison, a paired-sample t-test was used. For the consistency between the three methods, they were analyzed with regression and Pearson correlation coefficient. For determining accuracy, a receiver operating curve (ROC) was used. Bias error between the methods was determined through a Bland-Altman plot analysis. Our results did not show any significant statistical difference between ELISA and Cobas e411 (52.55 ± 7.4 µg/L vs. 52.58 ± 7.5 µg/L, p = 0.967), while there were significantly higher values from Cobas Integra 400 results than Cobas e411 (56.31 ± 7.8 µg/L vs. 52.58 ± 7.5 µg/L, p < 0.001) and ELISA (52.55 ± 7.4 µg/L vs. 56.31 ± 7.8 µg/L, p < 0.001). According to the correlation coefficient and linear regression analysis, a strong association between ELISA with Cobas e411 (r = 0.993, p < 0.001) and Cobas Integra 400 results (r = 0.994, p < 0.001) were revealed. For determining accuracy, Cobas e411 and Cobas Integra 400 results showed higher sensitivity (92.0%; 90.0%) and specificity (97.7%; 99.9%) respectively. Additionally, the Bland-Altman plot analysis showed a high agreement between the ELISA and Cobas e411 methods (bias -0.035). In contrast, there was a low agreement between the ELISA and Cobas Integra 400 methods (bias -3.75). Similarly, the agreement between Cobas e411 and Cobas Integra 400 methods was low (bias -3.72). Serum ferritin levels were measured by Cobas e411, and Cobas Integra 400 methods were strongly correlated with the ELISA results, with higher sensitivity, specificity, and accuracy. However, further investigations with larger samples are required for improved accuracy and more precise results, and to determine whether they can be used interchangeably.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Tipo de estudio: Observational_studies / Risk_factors_studies Idioma: En Revista: Diagnostics (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Yemen

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Tipo de estudio: Observational_studies / Risk_factors_studies Idioma: En Revista: Diagnostics (Basel) Año: 2022 Tipo del documento: Article País de afiliación: Yemen