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Sympathetic Pathways Target Cholinergic Neurons in the Human Colonic Myenteric Plexus.
Parker, Dominic R; Wiklendt, Lukasz; Humenick, Adam; Chen, Bao Nan; Sia, Tiong Cheng; Wattchow, David A; Dinning, Phil G; Brookes, Simon J H.
Afiliación
  • Parker DR; Laboratory of Neurogastroenterology, College of Medicine and Public Health, Flinders University, Adelaide, SA, Australia.
  • Wiklendt L; Colorectal Surgical Unit, Division of Surgery, Flinders Medical Centre, Bedford Park, SA, Australia.
  • Humenick A; Laboratory of Neurogastroenterology, College of Medicine and Public Health, Flinders University, Adelaide, SA, Australia.
  • Chen BN; Laboratory of Neurogastroenterology, College of Medicine and Public Health, Flinders University, Adelaide, SA, Australia.
  • Sia TC; Laboratory of Neurogastroenterology, College of Medicine and Public Health, Flinders University, Adelaide, SA, Australia.
  • Wattchow DA; Colorectal Surgical Unit, Division of Surgery, Flinders Medical Centre, Bedford Park, SA, Australia.
  • Dinning PG; Laboratory of Neurogastroenterology, College of Medicine and Public Health, Flinders University, Adelaide, SA, Australia.
  • Brookes SJH; Colorectal Surgical Unit, Division of Surgery, Flinders Medical Centre, Bedford Park, SA, Australia.
Front Neurosci ; 16: 863662, 2022.
Article en En | MEDLINE | ID: mdl-35368277
ABSTRACT

Background:

The sympathetic nervous system inhibits human colonic motility largely by effects on enteric neurons. Noradrenergic axons, which branch extensively in the myenteric plexus, are integral to this modulatory role, but whether they contact specific types of enteric neurons is unknown. The purpose of this study was to determine the association of noradrenergic varicosities with types of enteric neurons.

Methods:

Human colonic tissue from seven patients was fixed and dissected prior to multi-layer immunohistochemistry for human RNA binding proteins C and D (HuC/D) (pan-neuronal cell body labelling), tyrosine hydroxylase (TH, catecholaminergic labelling), Enkephalin (ENK), choline acetyltransferase (ChAT, cholinergic labelling) and/or nitric oxide synthase (NOS, nitrergic labelling) and imaged using confocal microscopy. TH-immunoreactive varicose nerve endings and myenteric cell bodies were reconstructed as three dimensional digital images. Data was exported to a purpose-built software package which quantified the density of varicosities close to the surface of each myenteric cell body.

Results:

TH-immunoreactive varicosities had a greater mean density within 1 µm of the surface of ChAT +/NOS- nerve cell bodies compared with ChAT-/NOS + cell bodies. Similarly, ENK-immunoreactive varicosities also had a greater mean density close to ChAT +/NOS- cell bodies compared with ChAT-/NOS + cells.

Conclusion:

A method for quantifying close associations between varicosities and nerve cell bodies was developed. Sympathetic axons in the myenteric plexus preferentially target cholinergic excitatory cells compared to nitrergic neurons (which are largely inhibitory). This connectivity is likely to be involved in inhibitory modulation of human colonic motility by the sympathetic nervous system.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: Front Neurosci Año: 2022 Tipo del documento: Article País de afiliación: Australia

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: Front Neurosci Año: 2022 Tipo del documento: Article País de afiliación: Australia