A dual-mode optical assay for iron(II) and gallic acid based on Fenton reaction.
Luminescence
; 38(7): 1167-1174, 2023 Jul.
Article
en En
| MEDLINE
| ID: mdl-35417927
ABSTRACT
The hydroxyl radicals (·OH) produced by the Fenton reaction of iron(II) and hydrogen peroxide (H2 O2 ) can oxidize the colorless 3,3',5,5'-tetramethylbenzidine (TMB) to blue oxidized TMB (Ox-TMB), resulting in a decrease in the fluorescence intensity of the reaction system and an increase in ultraviolet absorption. Ox-TMB had a visible absorption peak at 625 nm and a fluorescence peak around 420 nm. When gallic acid (GA) was added to the system, Ox-TMB was reduced to TMB, which made the color of the system disappear and the fluorescence recover. The linear ranges for determination of iron(II) were 0.5-10 µM (fluorometric) and 0.5-20 µM (colorimetric), and the detection limits were 0.25 µM (fluorometric) and 0.28 µM (colorimetric). The linear ranges for determination of GA were 0-80 µM (fluorometric) and 0-60 µM (colorimetric), and the detection limits were 0.31 µM (fluorometric) and 0.8 µM (colorimetric). The results of anti-interference experiments shew that this dual-mode assay had very good selectivity for the determination of iron(II) and GA.
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Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Ácido Gálico
/
Hierro
Idioma:
En
Revista:
Luminescence
Asunto de la revista:
BIOFISICA
/
BIOQUIMICA
Año:
2023
Tipo del documento:
Article