Intrafollicular Concentrations of the Oocyte-secreted Factors GDF9 and BMP15 Vary Inversely in Polycystic Ovaries.
J Clin Endocrinol Metab
; 107(8): e3374-e3383, 2022 07 14.
Article
en En
| MEDLINE
| ID: mdl-35511085
ABSTRACT
CONTEXT The oocyte-secreted factors growth differentiation factor 9 (GDF9) and bone morphogenetic protein 15 (BMP15) play essential roles in follicle development and oocyte maturation, and aberrant regulation might contribute to the pathogenesis of polycystic ovary syndrome. OBJECTIVE:
Are there measurable differences in concentrations of GDF9, BMP15, and the GDF9/BMP15 heterodimer in small antral follicle fluids from women with and without polycystic ovaries (PCO)? DESIGN ANDSETTING:
Follicle fluids (nâ =â 356) were collected from 4- to 11-mm follicles in unstimulated ovaries of 87 women undergoing ovarian tissue cryopreservation for fertility preservation. PATIENTS Twenty-seven women with PCO were identified and 60 women without PCO-like characteristics (non-PCO women) were matched according to age and follicle size. MAIN OUTCOMEMEASURES:
Intrafollicular concentrations of GDF9, BMP15, GDF9/BMP15 heterodimer, anti-Mullerian hormone (AMH), inhibin-A and -B, total inhibin, activin-B and -AB, and follistatin were measured using enzyme-linked immunosorbent assays.RESULTS:
The detectability of GDF9, BMP15, and the GDF9/BMP15 heterodimer were 100%, 94.4%, and 91.5%, respectively, and concentrations were significantly negatively correlated with increasing follicle size (Pâ <â 0.0001). GDF9 was significantly higher in women with PCO (PCO 4230â ±â 189 pg/mL [meanâ ±â SEM], nâ =â 188; non-PCO 3498â ±â 199 pg/mL, nâ =â 168; Pâ <â 0.03), whereas BMP15 was lower in women with PCO (PCO 431â ±â 40 pg/mL, nâ =â 125; non-PCO 573â ±â 55 pg/mL, nâ =â 109; Pâ =â 0.10), leading to a significantly higher GDF9BMP15 ratio in women with PCO (Pâ <â 0.01). Significant positive associations between BMP15 and AMH, activins, and inhibins in non-PCO women switched to negative associations in women with PCO.CONCLUSIONS:
Intrafollicular concentrations of GDF9 and BMP15 varied inversely in women with PCO reflecting an aberrant endocrine environment. An increased GDF9BMP15 ratio may be a new biomarker for PCO.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Oocitos
/
Síndrome del Ovario Poliquístico
/
Líquido Folicular
/
Proteína Morfogenética Ósea 15
/
Factor 9 de Diferenciación de Crecimiento
Tipo de estudio:
Diagnostic_studies
/
Prognostic_studies
Límite:
Female
/
Humans
Idioma:
En
Revista:
J Clin Endocrinol Metab
Año:
2022
Tipo del documento:
Article
País de afiliación:
Dinamarca