Investigation of HER-3 gene expression under the influence of carbohydrate biopolymers extract of shiitake and reishi in MCF-7 cell line.

INTRODUCTION: Edible-medicinal fungi are mainly used in Asian countries to prevent various diseases. These mushrooms are also used to treat lung diseases and cancer. Ganoderma lucidum and Lentinula edodes are the most important edible-medicinal fungi. The polysaccharides of these fungi are one of the bioactive compounds with anti-cancer properties. OBJECTIVE: Evaluation of anti-cancer effects of Shiitake and Reishi polysaccharides. METHODS: In this study, fungal polysaccharides were extracted using the hot water method and were purified by Diethylaminoethyl Sephadex A-25 (DEAE-Sephadex A-25) chromatography column and their concentration was measured by phenolic sulfuric acid method. The biological effects of the extracted polysaccharides from Ganoderma lucidum and Lentinula edodes on the MCF-7 cell line were investigated using an MTT assay and then its effects on the expression of the P53 cancer regulatory gene and HER-3 gene were investigated. RESULTS: Based on the results, the concentration of Ganoderma lucidum and Lentinula edodes extracted polysaccharides were 0.024 and 0.103 mg/ml, respectively. Polysaccharides of these two fungi increased the expression of the P53 gene and decreased the expression of the HER-3 gene in a dose and time-dependent manner. DISCUSSION: Natural biocompatible polysaccharides with anti-cancer properties that are native, are available, and inexpensive, so they can be used as dietary supplements to prevent and help treat cancer.