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Single-Cell RNA-Seq Identifies Dynamic Cardiac Transition Program from ADCs Induced by Leukemia Inhibitory Factor.
Yao, Jiayi; Ma, Feiyang; Zhang, Li; Zhu, Ching; Jumabay, Medet; Yao, Zehao; Wang, Lumin; Cai, Xinjiang; Zhang, Daoqin; Qiao, Xiaojing; Shivkumar, Kalyanam; Pellegrini, Matteo; Yao, Yucheng; Wu, Xiuju; Boström, Kristina I.
Afiliación
  • Yao J; Division of Cardiology, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA, USA.
  • Ma F; Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA, USA.
  • Zhang L; Chongqing International Institute for Immunology, Chongqing, People's Republic of China.
  • Zhu C; Division of Cardiology, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA, USA.
  • Jumabay M; Division of Cardiology, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA, USA.
  • Yao Z; Division of Allergy, Immunology Center for Immunity, Infection, and Inflammation Pediatrics, Dept of Medicine, University of California, San Diego, San Diego, CA, USA.
  • Wang L; Peking Union Medical College, Chinese Academy of Medical Science & Peking Union Medical College, Beijing, People's Republic of China.
  • Cai X; Institute of Precision Medicine, the Second Affiliated Hospital of Xi'an Jiaotong University, Xi'an, People's Republic of China.
  • Zhang D; Division of Cardiology, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA, USA.
  • Qiao X; Division of Cardiology, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA, USA.
  • Shivkumar K; Division of Cardiology, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA, USA.
  • Pellegrini M; Division of Cardiology, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA, USA.
  • Yao Y; Molecular Biology Institute, University of California, Los Angeles, Los Angeles, CA, USA.
  • Wu X; Dept of Molecular, Cell and Developmental Biology, University of California, Los Angeles, Los Angeles, CA, USA.
  • Boström KI; Division of Cardiology, David Geffen School of Medicine at University of California, Los Angeles, Los Angeles, CA, USA.
Stem Cells ; 40(10): 932-948, 2022 10 21.
Article en En | MEDLINE | ID: mdl-35896368
ABSTRACT
Adipose-derived cells (ADCs) from white adipose tissue are promising stem cell candidates because of their large regenerative reserves and the potential for cardiac regeneration. However, given the heterogeneity of ADC and its unsolved mechanisms of cardiac acquisition, ADC-cardiac transition efficiency remains low. In this study, we explored the heterogeneity of ADCs and the cellular kinetics of 39,432 single-cell transcriptomes along the leukemia inhibitory factor (LIF)-induced ADC-cardiac transition. We identified distinct ADC subpopulations that reacted differentially to LIF when entering the cardiomyogenic program, further demonstrating that ADC-myogenesis is time-dependent and initiates from transient changes in nuclear factor erythroid 2-related factor 2 (Nrf2) signaling. At later stages, pseudotime analysis of ADCs navigated a trajectory with 2 branches corresponding to activated myofibroblast or cardiomyocyte-like cells. Our findings offer a high-resolution dissection of ADC heterogeneity and cell fate during ADC-cardiac transition, thus providing new insights into potential cardiac stem cells.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Miocitos Cardíacos / Factor 2 Relacionado con NF-E2 Tipo de estudio: Prognostic_studies Idioma: En Revista: Stem Cells Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos
Texto completo
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Buscar en Google

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Miocitos Cardíacos / Factor 2 Relacionado con NF-E2 Tipo de estudio: Prognostic_studies Idioma: En Revista: Stem Cells Año: 2022 Tipo del documento: Article País de afiliación: Estados Unidos