H-NS Represses Biofilm Formation and c-di-GMP Synthesis in Vibrio parahaemolyticus.
Biomed Environ Sci
; 35(9): 821-829, 2022 Sep 20.
Article
en En
| MEDLINE
| ID: mdl-36189997
ABSTRACT
Objective:
This study aimed to investigate the regulation of histone-like nucleoid structuring protein (H-NS) on biofilm formation and cyclic diguanylate (c-di-GMP) synthesis in Vibrio parahaemolyticus RIMD2210633.Methods:
Regulatory mechanisms were analyzed by the combined utilization of crystal violet staining, quantification of c-di-GMP, quantitative real-time polymerase chain reaction, LacZ fusion, and electrophoretic-mobility shift assay.Results:
The deletion of hns enhanced the biofilm formation and intracellular c-di-GMP levels in V. parahaemolyticus RIMD2210633. H-NS can bind the upstream promoter-proximal DNA regions of scrA, scrG, VP0117, VPA0198, VPA1176, VP0699, and VP2979 to repress their transcription. These genes encode a group of proteins with GGDEF and/or EAL domains associated with c-di-GMP metabolism.Conclusion:
One of the mechanisms by which H-NS represses the biofilm formation by V. parahaemolyticus RIMD2210633 may be via repression of the production of intracellular c-di-GMP.Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Vibrio parahaemolyticus
Idioma:
En
Revista:
Biomed Environ Sci
Asunto de la revista:
SAUDE AMBIENTAL
Año:
2022
Tipo del documento:
Article
País de afiliación:
China