Fast backward steps and detachment of single kinesin molecules measured under a wide range of loads.
Traffic
; 24(10): 463-474, 2023 10.
Article
en En
| MEDLINE
| ID: mdl-37679870
ABSTRACT
To understand force generation under a wide range of loads, the stepping of single kinesin molecules was measured at loads from -20 to 42 pN by optical tweezers with high temporal resolution. The optical trap has been improved to halve positional noise and increase bandwidth by using 200-nm beads. The step size of the forward and backward steps was 8.2 nm even over a wide range of loads. Histograms of the dwell times of backward steps and detachment fit well to two independent exponential equations with fast (~0.4 ms) and slow (>3 ms) time constants, indicating the existence of a fast step in addition to the conventional slow step. The dwell times of the fast steps were almost independent of the load and ATP concentration, while those of the slow backward steps and detachment depended on those. We constructed the kinetic model to explain the fast and slow steps under a wide range of loads.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Cinesinas
Idioma:
En
Revista:
Traffic
Asunto de la revista:
FISIOLOGIA
Año:
2023
Tipo del documento:
Article
País de afiliación:
Japón