From fertilised oocyte to cultivated meat - harnessing bovine embryonic stem cells in the cultivated meat industry.
Reprod Fertil Dev
; 36(2): 124-132, 2023 Dec.
Article
en En
| MEDLINE
| ID: mdl-38064188
ABSTRACT
Global demand for animal protein is on the rise, but many practices common in conventional production are no longer scalable due to environmental impact, public health concerns, and fragility of food systems. For these reasons and more, a pressing need has arisen for sustainable, nutritious, and animal welfare-conscious sources of protein, spurring research dedicated to the production of cultivated meat. Meat mainly consists of muscle, fat, and connective tissue, all of which can be sourced and differentiated from pluripotent stem cells to resemble their nutritional values in muscle tissue. In this paper, we outline the approach that we took to derive bovine embryonic stem cell lines (bESCs) and to characterise them using FACS (fluorescence-activated cell sorting), real-time PCR and immunofluorescence staining. We show their cell growth profile and genetic stability and demonstrate their induced differentiation to mesoderm committed cells. In addition, we discuss our strategy for preparation of master and working cell banks, by which we can expand and grow cells in suspension in quantities suitable for mass production. Consequently, we demonstrate the potential benefits of harnessing bESCs in the production of cultivated meat.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Técnicas de Cultivo de Célula
Límite:
Animals
Idioma:
En
Revista:
Reprod Fertil Dev
Asunto de la revista:
MEDICINA REPRODUTIVA
Año:
2023
Tipo del documento:
Article