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Genome-wide association study reveals serovar-associated genetic loci in Riemerella anatipestifer.
Yang, Zhishuang; Yang, Xueqin; Wang, Mingshu; Jia, Renyong; Chen, Shun; Liu, Mafeng; Zhao, Xinxin; Yang, Qiao; Wu, Ying; Zhang, Shaqiu; Huang, Juan; Ou, Xumin; Mao, Sai; Gao, Qun; Sun, Di; Tian, Bin; Zhu, Dekang; Cheng, Anchun.
Afiliación
  • Yang Z; Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China.
  • Yang X; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China.
  • Wang M; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, Sichuan, China.
  • Jia R; Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China.
  • Chen S; Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China.
  • Liu M; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China.
  • Zhao X; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, Sichuan, China.
  • Yang Q; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, Sichuan, China.
  • Wu Y; Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China.
  • Zhang S; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China.
  • Huang J; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, Sichuan, China.
  • Ou X; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, Sichuan, China.
  • Mao S; Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China.
  • Gao Q; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China.
  • Sun D; International Joint Research Center for Animal Disease Prevention and Control of Sichuan Province, Chengdu, Sichuan, China.
  • Tian B; Engineering Research Center of Southwest Animal Disease Prevention and Control Technology, Ministry of Education of the People's Republic of China, Chengdu, Sichuan, China.
  • Zhu D; Research Center of Avian Diseases, College of Veterinary Medicine, Sichuan Agricultural University, Chengdu, Sichuan, China.
  • Cheng A; Key Laboratory of Animal Disease and Human Health of Sichuan Province, Chengdu, Sichuan, China.
BMC Genomics ; 25(1): 57, 2024 Jan 13.
Article en En | MEDLINE | ID: mdl-38216873
ABSTRACT

BACKGROUND:

The disease caused by Riemerella anatipestifer (R. anatipestifer, RA) results in large economic losses to the global duck industry every year. Serovar-related genomic variation, such as the O-antigen and capsular polysaccharide (CPS) gene clusters, has been widely used for serotyping in many gram-negative bacteria. RA has been classified into at least 21 serovars based on slide agglutination, but the molecular basis of serotyping is unknown. In this study, we performed a pan-genome-wide association study (Pan-GWAS) to identify the genetic loci associated with RA serovars.

RESULTS:

The results revealed a significant association between the putative CPS synthesis gene locus and the serological phenotype. Further characterization of the CPS gene clusters in 11 representative serovar strains indicated that they were highly diverse and serovar-specific. The CPS gene cluster contained the key genes wzx and wzy, which are involved in the Wzx/Wzy-dependent pathway of CPS synthesis. Similar CPS loci have been found in some other species within the family Weeksellaceae. We have also shown that deletion of the wzy gene in RA results in capsular defects and cross-agglutination.

CONCLUSIONS:

This study indicates that the CPS synthesis gene cluster of R. anatipestifer is a serotype-specific genetic locus. Importantly, our finding provides a new perspective for the systematic analysis of the genetic basis of the R anatipestifer serovars and a potential target for establishing a complete molecular serotyping scheme.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Enfermedades de las Aves de Corral / Riemerella Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Animals Idioma: En Revista: BMC Genomics Asunto de la revista: GENETICA Año: 2024 Tipo del documento: Article País de afiliación: China

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Enfermedades de las Aves de Corral / Riemerella Tipo de estudio: Prognostic_studies / Risk_factors_studies Límite: Animals Idioma: En Revista: BMC Genomics Asunto de la revista: GENETICA Año: 2024 Tipo del documento: Article País de afiliación: China