Integrated conjugal plasmid pLS20 in the Bacillus subtilis genome produced 850-kbp circular subgenomes transmissible to another B. subtilis.

Itaya, Mitsuhiro; Kataoka, Masakazu

Itaya, Mitsuhiro; Kataoka, Masakazu.

Afiliación

Itaya M; Department of Biomedical Engineering Graduate School of Science and Technology, Shinshu University, Nagano, Japan.
Kataoka M; Department of Biomedical Engineering Graduate School of Science and Technology, Shinshu University, Nagano, Japan.

Genes Cells ; 29(7): 584-588, 2024 Jul.

Article en En | MEDLINE | ID: mdl-38660704

ABSTRACT

ABSTRACT

Bacillus subtilis was engineered to produce circular subgenomes that are directly transmittable to another B. subtilis. The conjugational plasmid pLS20 integrated into the B. subtilis genome supported not only subgenome replication but also transmission to another B. subtilis species. The subgenome system developed in this study completes a streamlined platform from the synthesis to the transmission of giant DNA by B. subtilis.

Asunto(s)

Bacillus subtilis; Genoma Bacteriano; Plásmidos; Bacillus subtilis/genética; Bacillus subtilis/metabolismo; Plásmidos/genética; ADN Circular/genética; ADN Circular/metabolismo; Conjugación Genética; Replicación del ADN/genética; ADN Bacteriano/genética; ADN Bacteriano/metabolismo

Palabras clave

B. Subtilis; conjugational plasmid; genome synthesis; pLS20; subgenomes

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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Asunto principal: Plásmidos / Bacillus subtilis / Genoma Bacteriano Idioma: En Revista: Genes Cells Asunto de la revista: BIOLOGIA MOLECULAR Año: 2024 Tipo del documento: Article País de afiliación: Japón

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