Protocol for transducing human primary epithelial prostate cells and patient-derived organoids with high efficiency.
STAR Protoc
; 5(3): 103200, 2024 Sep 20.
Article
en En
| MEDLINE
| ID: mdl-39028619
ABSTRACT
Patient-derived organoids (PDOs) are now used to study many diseases, including prostate cancer. Here, we present a protocol for the transduction of human epithelial prostate cells and PDOs. We describe the steps for producing lentiviruses and transducing PDOs with high efficiency to obtain either overexpression or knockdown of specific genes. More generally, this protocol represents an efficient lentiviral transduction technique to study cell biology using various organoid models.
Palabras clave
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Próstata
/
Organoides
/
Lentivirus
/
Células Epiteliales
Límite:
Humans
/
Male
Idioma:
En
Revista:
STAR Protoc
Año:
2024
Tipo del documento:
Article
País de afiliación:
Canadá