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Inconsistent Transcriptomic Responses to Hexabromocyclododecane in Japanese Quail: A Comparative Analysis of Results From Four Different Study Designs.
Béziers, Paul; Legrand, Elena; Boulanger, Emily; Basu, Niladri; Ewald, Jessica D; Henry, Paula; Hecker, Markus; Xia, Jianguo; Karouna-Renier, Natalie; Crump, Doug; Head, Jessica.
Afiliación
  • Béziers P; Faculty of Agricultural and Environmental Sciences, McGill University, Montreal, Québec, Canada.
  • Legrand E; Faculty of Agricultural and Environmental Sciences, McGill University, Montreal, Québec, Canada.
  • Boulanger E; Faculty of Agricultural and Environmental Sciences, McGill University, Montreal, Québec, Canada.
  • Basu N; Faculty of Agricultural and Environmental Sciences, McGill University, Montreal, Québec, Canada.
  • Ewald JD; Faculty of Agricultural and Environmental Sciences, McGill University, Montreal, Québec, Canada.
  • Henry P; U.S. Geological Survey, Eastern Ecological Science Center at Patuxent Research Refuge, Laurel, Maryland, USA.
  • Hecker M; School of the Environment and Sustainability and Toxicology Centre, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
  • Xia J; Faculty of Agricultural and Environmental Sciences, McGill University, Montreal, Québec, Canada.
  • Karouna-Renier N; U.S. Geological Survey, Eastern Ecological Science Center at Patuxent Research Refuge, Laurel, Maryland, USA.
  • Crump D; National Wildlife Research Center, Environment and Climate Change Canada, Ottawa, Ontario, Canada.
  • Head J; Faculty of Agricultural and Environmental Sciences, McGill University, Montreal, Québec, Canada.
Environ Toxicol Chem ; 2024 Jul 29.
Article en En | MEDLINE | ID: mdl-39073395
ABSTRACT
Efforts to use transcriptomics for toxicity testing have classically relied on the assumption that chemicals consistently produce characteristic transcriptomic signatures that are reflective of their mechanism of action. However, the degree to which transcriptomic responses are conserved across different test methodologies has seldom been explored. With increasing regulatory demand for New Approach Methods (NAMs) that use alternatives to animal models and high-content approaches such as transcriptomics, this type of comparative analysis is needed. We examined whether common genes are dysregulated in Japanese quail (Coturnix japonica) liver following sublethal exposure to the flame retardant hexabromocyclododecane (HBCD), when life stage and test methodologies differ. The four exposure scenarios included one NAM Study 1-early-life stage (ELS) exposure via a single egg injection, and three more traditional approaches; Study 2-adult exposure using a single oral gavage; Study 3-ELS exposure via maternal deposition after adults were exposed through their diet for 7 weeks; and Study 4-ELS exposure via maternal deposition and re-exposure of nestlings through their diet for 17 weeks. The total number of differentially expressed genes (DEGs) detected in each study was variable (Study 1, 550; Study 2, 192; Study 3, 1; Study 4, 3) with only 19 DEGs shared between Studies 1 and 2. Factors contributing to this lack of concordance are discussed and include differences in dose, but also quail strain, exposure route, sampling time, and HBCD stereoisomer composition. The results provide a detailed overview of the transcriptomic responses to HBCD at different life stages and routes of exposure in a model avian species and highlight certain challenges and limits of comparing transcriptomics across different test methodologies. Environ Toxicol Chem 2024;001-11. © 2024 The Author(s). Environmental Toxicology and Chemistry published by Wiley Periodicals LLC on behalf of SETAC.
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Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: Environ Toxicol Chem Año: 2024 Tipo del documento: Article País de afiliación: Canadá

Texto completo: 1 Colección: 01-internacional Banco de datos: MEDLINE Idioma: En Revista: Environ Toxicol Chem Año: 2024 Tipo del documento: Article País de afiliación: Canadá