Light chain germ-line genes and the immune response to 2-phenyloxazolone.
EMBO J
; 4(13A): 3439-45, 1985 Dec 16.
Article
en En
| MEDLINE
| ID: mdl-3937727
ABSTRACT
Direct sequencing of mRNA has shown that the early primary response of the BALB/c mouse to the hapten 2-phenyloxazolone is dominated by antibodies with a particular light chain, V kappa-Ox1. Although the V kappa-Ox1 sequence is still commonly expressed later in the response it now includes a number of nucleotide changes. From two independent BALB/c germ-line DNA libraries 13 different genes hybridizing to a V kappa-Ox1 probe were isolated and characterized. Two are identical to mRNA sequences found in the early primary response, one of which is the V kappa-Ox1 sequence. None of the germ-line clones show the characteristic nucleotide changes contained in the late anti-phenyloxazolone light chain mRNAs. These results demonstrate that the V kappa-Ox1 sequence used in the early primary response is entirely encoded by the germ-line and further substantiate the importance of somatic mutations in the maturation of the anti-phenyloxazolone response. The statistical analysis of the data shows that the V kappa-Ox1 related germ-line gene family contains greater than 20 and probably less than 50 genes.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Oxazoles
/
Región Variable de Inmunoglobulina
/
Oxazolona
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Cadenas kappa de Inmunoglobulina
/
Diversidad de Anticuerpos
Límite:
Animals
Idioma:
En
Revista:
EMBO J
Año:
1985
Tipo del documento:
Article