Selective translational regulation of ribosomal protein gene expression during early development of Drosophila melanogaster.
Mol Cell Biol
; 5(12): 3583-92, 1985 Dec.
Article
en En
| MEDLINE
| ID: mdl-3939320
ABSTRACT
We have previously characterized a cloned cDNA coding for a developmentally regulated mRNA in Drosophila melanogaster whose expression is selectively regulated at the translational level during oogenesis and embryogenesis. In this report we show that this translationally regulated mRNA (rpA1) codes for an acidic ribosomal protein. Furthermore, our results indicate that most ribosomal protein mRNAs are regulated similarly to rpA1 mRNA. This conclusion is based on cell-free translation of mRNAs derived from polysomes and postpolysomal supernatants as well as in vivo labeling experiments. Thus, the translation of many ribosomal protein mRNAs appears to be temporally related to the synthesis of rRNA during D. melanogaster development. The relationship between rRNA transcription and ribosomal protein mRNA translation was further investigated by genetically reducing rRNA synthesis with the use of bobbed mutants. Unexpectedly, neither ribosomal protein mRNA abundance nor translation was altered in these mutants.
Texto completo:
1
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Proteínas Ribosómicas
/
Biosíntesis de Proteínas
/
Regulación de la Expresión Génica
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Drosophila melanogaster
Límite:
Animals
Idioma:
En
Revista:
Mol Cell Biol
Año:
1985
Tipo del documento:
Article