Electroneutral Na+/dicarboxylic amino acid cotransport in rat intestinal brush border membrane vesicles.
Biochim Biophys Acta
; 689(1): 97-105, 1982 Jul 14.
Article
en En
| MEDLINE
| ID: mdl-6125215
ABSTRACT
L-Glutamate and L-aspartate transport into osmotically active intestinal brush border membrane vesicles is specifically increased by Na+ gradient (extravesicular greater than intravesicular) which in addition energizes the transient accumulation (overshoot) of the two amino acids against their concentration gradients. The "overshoot" is observed at minimal external Na+ concentration of 100 mM for L-glutamate and 60 mM for L-aspartate; saturation with respect to [Na+] was observed at a concentration near 100 mM for both amino acids. Increasing amino acid concentration, saturation of the uptake rate was observed for L-glutamate and L-aspartate in the concentration range between 1 and 2 mM. Experiments showing mutual inhibition and transtimulation of the two amino acids indicate that the same Na+ -dependent transport system is shared by the two acidic amino acids. The imposition of diffusion potentials across the membrane vesicles artificially induced by addition of valinomycin in the presence of a K+ gradient supports the conclusion that the cotransport Na+/dicarboxylic amino acid in rat brush border membrane vesicles is electroneutral.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Sodio
/
Membrana Celular
/
Aminoácidos Dicarboxílicos
/
Intestino Delgado
/
Microvellosidades
Límite:
Animals
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1982
Tipo del documento:
Article