DNA cloning of Plasmodium falciparum circumsporozoite gene: amino acid sequence of repetitive epitope.
Science
; 225(4662): 628-30, 1984 Aug 10.
Article
en En
| MEDLINE
| ID: mdl-6204384
ABSTRACT
A clone of complementary DNA encoding the circumsporozoite (CS) protein of the human malaria parasite Plasmodium falciparum has been isolated by screening an Escherichia coli complementary DNA library with a monoclonal antibody to the CS protein. The DNA sequence of the complementary DNA insert encodes a four-amino acid sequence proline-asparagine-alanine-asparagine, tandemly repeated 23 times. The CS beta-lactamase fusion protein specifically binds monoclonal antibodies to the CS protein and inhibits the binding of these antibodies to native Plasmodium falciparum CS protein. These findings provide a basis for the development of a vaccine against Plasmodium falciparum malaria.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Plasmodium falciparum
/
Secuencias Repetitivas de Ácidos Nucleicos
/
Proteínas Protozoarias
/
Clonación Molecular
/
Genes
/
Epítopos
/
Antígenos de Superficie
Idioma:
En
Revista:
Science
Año:
1984
Tipo del documento:
Article