The use of drugs which deplete intracellular glutathione in hypoxic cell radiosensitization.
Int J Radiat Oncol Biol Phys
; 8(3-4): 439-42, 1982.
Article
en En
| MEDLINE
| ID: mdl-7107365
ABSTRACT
Diethylmaleate (DEM) is a thiol-binding reagent with specificity toward glutathione. Treatment of Chinese hamster ovary (CHO) cells in vitro with 2 x 10(-4) M DEM for one hour results in a decrease in glutathione content to less than 5% of control, without cytotoxicity. This treatment results in dose-modifying sensitization to radiation under hypoxic conditions, with no effect on the shoulder of the radiation survival curve. No effect on the radiation sensitivity of oxygenated cells was seen. DEM pretreatment enhances the radiosensitization of hypoxic cells by misonidazole, as well. Similar results were obtained in vivo with EMT6 tumors in BALB/c mice. Analysis of DNA damage by the alkaline elution assay indicates that DEM enhances radiation-induced single-strand breaks, but does not significantly affect repair, while diamide and N-ethylmaleimide inhibit repair, in addition to enhancing radiation-induced single-strand breaks.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fármacos Sensibilizantes a Radiaciones
/
Glutatión
/
Maleatos
Límite:
Animals
Idioma:
En
Revista:
Int J Radiat Oncol Biol Phys
Año:
1982
Tipo del documento:
Article