Retroviral immortalization of phagocytic and dendritic cell clones as a tool to investigate functional heterogeneity.
J Immunol Methods
; 174(1-2): 269-79, 1994 Sep 14.
Article
en En
| MEDLINE
| ID: mdl-8083532
ABSTRACT
We have developed a method to generate immortalized phagocytic and dendritic cell clones from various mouse tissues such as spleen, thymus, brain and bone marrow. The clones were phenotypically characterized and shown to retain the ability to respond to immune or inflammatory signals, e.g., IFN-gamma. Functional cytokine activity and nitric oxide production were maintained in activated macrophages, microglial and dendritic cell clones. Immune functions, such as antigen presentation was exhibited by all clones whereas tissue-specific properties such as the ability to respond to corticotropin-releasing hormone and produce beta-endorphin was shown in microglial cell clones but not in macrophage cell clones, indicating that heterogeneity of cells of the mononuclear-phagocytic lineage can be maintained in vitro after the immortalization procedure. Moreover, the continuous proliferation of the clones could be inhibited by various stimuli and further differentiation of the cells could be achieved in vitro.
Buscar en Google
Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Fagocitos
/
Células Dendríticas
/
Transformación Celular Viral
Límite:
Animals
Idioma:
En
Revista:
J Immunol Methods
Año:
1994
Tipo del documento:
Article
País de afiliación:
Italia