Synthetic boundary cell in an analytical ultracentrifuge to determine the flotation coefficient of human serum lipoproteins.
Biochim Biophys Acta
; 1169(1): 103-6, 1993 Jul 21.
Article
en En
| MEDLINE
| ID: mdl-8334144
ABSTRACT
Human serum lipoproteins were analyzed by a new modified synthetic boundary cell using an analytical ultracentrifuge. This cell allows the formation of the synthetic boundary at the bottom level of the cell with self-adjusting meniscus and baseline. Thus, the total amount of lipoproteins was seen as a single peak at first. During centrifugation, each component of the lipoproteins was separated according to its flotation characteristics. It was, therefore, possible to determine precisely all lipoprotein components, especially high-density lipoproteins, in the presence of a more rapidly migrating species and to calculate the flotation coefficient of each lipoprotein using the formula for sedimentation coefficient reported by Svedberg (Svedberg, T. (1925) Kolloid-Z. 36, 53-64.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Ultracentrifugación
/
Lipoproteínas
Límite:
Humans
Idioma:
En
Revista:
Biochim Biophys Acta
Año:
1993
Tipo del documento:
Article
País de afiliación:
Japón