Sample self-stacking and sample stacking in zone electrophoresis with major sample components of like charge: general model and scheme of possible modes.
Electrophoresis
; 16(11): 2039-50, 1995 Nov.
Article
en En
| MEDLINE
| ID: mdl-8748734
ABSTRACT
A theoretical study is presented of zone electrophoretic behavior of samples that contain one or more minor analytes and at least one major ionogenic component of like charge. Based on a simple model comprising weak univalent anionic electrolytes, conditions are derived under which analytes are temporarily focused isotachophoretically into very narrow zones by a sample self-stacking effect provided by the major sample components. Requirements for minimal/maximal mobility and a background coion concentration dependent minimal concentration of a major sample component (stacker) are presented. For systems in which sample self-stacking does not apply, an expression for the concentrating factor is derived that involves the effects of both nonselective (classical) and selective sample stacking, the latter being a consequence of electrophoretic separation of the minor analyte from the major component. The theory derived is discussed with selected model examples by using both numerical calculation and computer simulation.
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Colección:
01-internacional
Banco de datos:
MEDLINE
Asunto principal:
Cómputos Matemáticos
/
Modelos Moleculares
/
Electroforesis Capilar
Tipo de estudio:
Prognostic_studies
Idioma:
En
Revista:
Electrophoresis
Año:
1995
Tipo del documento:
Article